A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer
Obesity is a global epidemic that has affected the lives of over 14% of adults worldwide and over a third of Americans. Obesity is associated with the increased risk of thirteen obesity-associated cancers and poor cancer outcomes. Bariatric surgery is the most effective method of sustained weight lo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2025.1432817/full |
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| author | Arvind V. Ramesh Sydney C. Joseph Margaret S. Bohm Emily W. Grey Joel H. Elasy Brianne M. Hibl Oluwatosin T. Asunloye Ki-Suk Kim Teri D. Doss Joseph F. Pierre Katherine L. Cook Liza Makowski Liza Makowski Liza Makowski Laura M. Sipe |
| author_facet | Arvind V. Ramesh Sydney C. Joseph Margaret S. Bohm Emily W. Grey Joel H. Elasy Brianne M. Hibl Oluwatosin T. Asunloye Ki-Suk Kim Teri D. Doss Joseph F. Pierre Katherine L. Cook Liza Makowski Liza Makowski Liza Makowski Laura M. Sipe |
| author_sort | Arvind V. Ramesh |
| collection | DOAJ |
| description | Obesity is a global epidemic that has affected the lives of over 14% of adults worldwide and over a third of Americans. Obesity is associated with the increased risk of thirteen obesity-associated cancers and poor cancer outcomes. Bariatric surgery is the most effective method of sustained weight loss and has been steadily increasing in clinical use over the past 4 decades. Importantly, bariatric surgery is established to decrease cancer risk. Vertical sleeve gastrectomy (VSG) is currently the most common bariatric surgery procedure. To evaluate underlying mechanisms of bariatric associated cancer protection, we developed a robust pre-clinical model of bariatric surgery-induced weight loss in mice. Using multiple strains, we established detailed procedures, defined best practices, and noted specific controls to include to examine mediators critical to cancer onset. This VSG protocol includes stringent pre- and post-operational measures to reduce stress-associated weight loss in obese mice to achieve rigorous and reproducible bariatric surgery-associated weight loss. In addition, we describe collection of fecal and intestinal samples as well as Peyer’s patches as important mediators of bariatric surgery’s impact on cancer risk. In conclusion, as obesity and weight loss approaches including bariatric surgery are increasingly examined in cancer risk and outcomes including immunotherapy, the establishment of robust pre-clinical interventions will allow the field to address critical underlying mechanisms mediating the benefits of weight loss and cancer. |
| format | Article |
| id | doaj-art-34a01de0b8b94f9e99aa8c740e7cc7f0 |
| institution | Matheson Library |
| issn | 2813-4338 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj-art-34a01de0b8b94f9e99aa8c740e7cc7f02025-07-15T13:24:22ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382025-07-01410.3389/frmbi.2025.14328171432817A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancerArvind V. Ramesh0Sydney C. Joseph1Margaret S. Bohm2Emily W. Grey3Joel H. Elasy4Brianne M. Hibl5Oluwatosin T. Asunloye6Ki-Suk Kim7Teri D. Doss8Joseph F. Pierre9Katherine L. Cook10Liza Makowski11Liza Makowski12Liza Makowski13Laura M. Sipe14Division of Hematology and Oncology, Department of Medicine, College of Medicine, University of Tennessee Health Science Center (UTHSC), Memphis, TN, United StatesDivision of Hematology and Oncology, Department of Medicine, College of Medicine, University of Tennessee Health Science Center (UTHSC), Memphis, TN, United StatesDepartment of Microbiology, Immunology, and Biochemistry, College of Medicine, UTHSC, Memphis, TN, United StatesDivision of Hematology and Oncology, Department of Medicine, College of Medicine, University of Tennessee Health Science Center (UTHSC), Memphis, TN, United StatesDivision of Hematology and Oncology, Department of Medicine, College of Medicine, University of Tennessee Health Science Center (UTHSC), Memphis, TN, United StatesOffice of Comparative Medicine, University of Utah, Salt Lake City, UT, United StatesDepartment of Physiology, College of Medicine, UTHSC, Memphis, TN, United StatesDepartment of Physiology, College of Medicine, UTHSC, Memphis, TN, United StatesDepartment of Molecular Physiology and Biophysics, Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University, Nashville, TN, United StatesDepartment of Nutritional Sciences, College of Agriculture and Life Science, The University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Nutritional Sciences, College of Agriculture and Life Science, The University of Wisconsin-Madison, Madison, WI, United StatesDivision of Hematology and Oncology, Department of Medicine, College of Medicine, University of Tennessee Health Science Center (UTHSC), Memphis, TN, United StatesDepartment of Microbiology, Immunology, and Biochemistry, College of Medicine, UTHSC, Memphis, TN, United StatesUTHSC Center for Cancer Research, College of Medicine, UTHSC, Memphis, TN, United StatesDepartment of Biology, University of Mary Washington, Fredericksburg, VA, United StatesObesity is a global epidemic that has affected the lives of over 14% of adults worldwide and over a third of Americans. Obesity is associated with the increased risk of thirteen obesity-associated cancers and poor cancer outcomes. Bariatric surgery is the most effective method of sustained weight loss and has been steadily increasing in clinical use over the past 4 decades. Importantly, bariatric surgery is established to decrease cancer risk. Vertical sleeve gastrectomy (VSG) is currently the most common bariatric surgery procedure. To evaluate underlying mechanisms of bariatric associated cancer protection, we developed a robust pre-clinical model of bariatric surgery-induced weight loss in mice. Using multiple strains, we established detailed procedures, defined best practices, and noted specific controls to include to examine mediators critical to cancer onset. This VSG protocol includes stringent pre- and post-operational measures to reduce stress-associated weight loss in obese mice to achieve rigorous and reproducible bariatric surgery-associated weight loss. In addition, we describe collection of fecal and intestinal samples as well as Peyer’s patches as important mediators of bariatric surgery’s impact on cancer risk. In conclusion, as obesity and weight loss approaches including bariatric surgery are increasingly examined in cancer risk and outcomes including immunotherapy, the establishment of robust pre-clinical interventions will allow the field to address critical underlying mechanisms mediating the benefits of weight loss and cancer.https://www.frontiersin.org/articles/10.3389/frmbi.2025.1432817/fullobesitybreast cancerPeyer’s patchanti-tumor immunitymicrobiota |
| spellingShingle | Arvind V. Ramesh Sydney C. Joseph Margaret S. Bohm Emily W. Grey Joel H. Elasy Brianne M. Hibl Oluwatosin T. Asunloye Ki-Suk Kim Teri D. Doss Joseph F. Pierre Katherine L. Cook Liza Makowski Liza Makowski Liza Makowski Laura M. Sipe A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer Frontiers in Microbiomes obesity breast cancer Peyer’s patch anti-tumor immunity microbiota |
| title | A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| title_full | A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| title_fullStr | A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| title_full_unstemmed | A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| title_short | A standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| title_sort | standardized method for vertical sleeve gastrectomy bariatric surgery investigations in cancer |
| topic | obesity breast cancer Peyer’s patch anti-tumor immunity microbiota |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2025.1432817/full |
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