DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam
Despite the rich diversity of marine gastropods, limited taxonomic investigations on edible species raise concerns regarding fisheries management and food safety in Vietnam. In this study, we employed iterative taxonomy by combining morphological identification and DNA barcoding using the COI, 12S-r...
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Elsevier
2025-12-01
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| Series: | Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666833525001510 |
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| author | Davin H.E. Setiamarga Moe Shimizu Satoko Nakashima Chihiro Osaki Kazuki Hirota Lukytawati Anggraeni Do Van Tu Takenori Sasaki |
| author_facet | Davin H.E. Setiamarga Moe Shimizu Satoko Nakashima Chihiro Osaki Kazuki Hirota Lukytawati Anggraeni Do Van Tu Takenori Sasaki |
| author_sort | Davin H.E. Setiamarga |
| collection | DOAJ |
| description | Despite the rich diversity of marine gastropods, limited taxonomic investigations on edible species raise concerns regarding fisheries management and food safety in Vietnam. In this study, we employed iterative taxonomy by combining morphological identification and DNA barcoding using the COI, 12S-rRNA, 18S-rRNA, 28S-rRNA, and histone H3 gene markers, to confirm the taxonomy of 126 marine gastropod museum specimens collected from various fish markets in Vietnam. Morphological identification assigned 113 of the 126 specimens to 53 species. Sequences were obtained for all samples, although not all genes were successfully sequenced for every specimen. When all gene markers were used, 58 % in 2023 and 79 % in 2025 of the 113 samples were identified at the species level (excluding the morphologically unidentified individuals), compared to only 51 % in 2023 and 62 % in 2025 when only COI was used. The higher match rates in 2025 likely reflect ongoing improvements in public reference databases. Phylogenetic and genetic distance analyses supported these results, revealing monophyletic species and genera. This study revealed that >50 species are part of local diets, emphasizing the importance of fundamental biodiversity studies, including alpha taxonomic surveys, for managing marine gastropod fisheries and highlighting marine gastropods’ potential as novel food resources. By utilizing vouchered museum specimens, this study also contributes to developing a reliable reference database for identification and monitoring edible marine gastropods in Vietnam and Southeast Asia. |
| format | Article |
| id | doaj-art-3238c0414b1042fbb7935d97b56433e7 |
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| issn | 2666-8335 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
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| series | Future Foods |
| spelling | doaj-art-3238c0414b1042fbb7935d97b56433e72025-07-08T04:04:52ZengElsevierFuture Foods2666-83352025-12-0112100689DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in VietnamDavin H.E. Setiamarga0Moe Shimizu1Satoko Nakashima2Chihiro Osaki3Kazuki Hirota4Lukytawati Anggraeni5Do Van Tu6Takenori Sasaki7Department of Applied Chemistry and Biochemistry, National Institute of Technology (KOSEN), Wakayama College, Gobo, 644-0023, Wakayama, Japan; Advance School for Ecosystem Engineering, National Institute of Technology (KOSEN), Wakayama College, Gobo 644-0023, Wakayama, Japan; Wakayama Prefectural Museum of Natural History, Kainan 642-0001, Wakayama, Japan; The University Museum, The University of Tokyo, Bunkyo-ku 113-0033, Tokyo, Japan; institute for coMmunity Innovation in coLLAboration with KOSEN (MILLA), Toyohashi University of Technology, Toyohashi 441-8580, Aichi, Japan; Faculty of Fisheries and Marine Sciences, IPB University, Bogor 16680, West Java, Indonesia; Corresponding author.Department of Applied Chemistry and Biochemistry, National Institute of Technology (KOSEN), Wakayama College, Gobo, 644-0023, Wakayama, Japan; Advance School for Ecosystem Engineering, National Institute of Technology (KOSEN), Wakayama College, Gobo 644-0023, Wakayama, Japan; Department of Earth and Planetary Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku 113-0033, Tokyo, JapanDepartment of Applied Chemistry and Biochemistry, National Institute of Technology (KOSEN), Wakayama College, Gobo, 644-0023, Wakayama, Japan; Advance School for Ecosystem Engineering, National Institute of Technology (KOSEN), Wakayama College, Gobo 644-0023, Wakayama, JapanDepartment of Applied Chemistry and Biochemistry, National Institute of Technology (KOSEN), Wakayama College, Gobo, 644-0023, Wakayama, Japan; Advance School for Ecosystem Engineering, National Institute of Technology (KOSEN), Wakayama College, Gobo 644-0023, Wakayama, JapanDepartment of Applied Chemistry and Biochemistry, National Institute of Technology (KOSEN), Wakayama College, Gobo, 644-0023, Wakayama, Japan; Department of Earth and Planetary Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku 113-0033, Tokyo, JapanDepartment of Economics, Faculty of Economics and Management, IPB University, Bogor 16680, West Java, IndonesiaInstitute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Ha Noi 100000, Vietnam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ha Noi 100000, VietnamThe University Museum, The University of Tokyo, Bunkyo-ku 113-0033, Tokyo, Japan; Department of Earth and Planetary Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku 113-0033, Tokyo, JapanDespite the rich diversity of marine gastropods, limited taxonomic investigations on edible species raise concerns regarding fisheries management and food safety in Vietnam. In this study, we employed iterative taxonomy by combining morphological identification and DNA barcoding using the COI, 12S-rRNA, 18S-rRNA, 28S-rRNA, and histone H3 gene markers, to confirm the taxonomy of 126 marine gastropod museum specimens collected from various fish markets in Vietnam. Morphological identification assigned 113 of the 126 specimens to 53 species. Sequences were obtained for all samples, although not all genes were successfully sequenced for every specimen. When all gene markers were used, 58 % in 2023 and 79 % in 2025 of the 113 samples were identified at the species level (excluding the morphologically unidentified individuals), compared to only 51 % in 2023 and 62 % in 2025 when only COI was used. The higher match rates in 2025 likely reflect ongoing improvements in public reference databases. Phylogenetic and genetic distance analyses supported these results, revealing monophyletic species and genera. This study revealed that >50 species are part of local diets, emphasizing the importance of fundamental biodiversity studies, including alpha taxonomic surveys, for managing marine gastropod fisheries and highlighting marine gastropods’ potential as novel food resources. By utilizing vouchered museum specimens, this study also contributes to developing a reliable reference database for identification and monitoring edible marine gastropods in Vietnam and Southeast Asia.http://www.sciencedirect.com/science/article/pii/S2666833525001510Edible marine gastropodsMuseomicsTraceabilityFood safetyAlternative protein sourceBiodiversity |
| spellingShingle | Davin H.E. Setiamarga Moe Shimizu Satoko Nakashima Chihiro Osaki Kazuki Hirota Lukytawati Anggraeni Do Van Tu Takenori Sasaki DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam Future Foods Edible marine gastropods Museomics Traceability Food safety Alternative protein source Biodiversity |
| title | DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam |
| title_full | DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam |
| title_fullStr | DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam |
| title_full_unstemmed | DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam |
| title_short | DNA barcoding of museum-vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in Vietnam |
| title_sort | dna barcoding of museum vouchered samples collected from fish markets reveals an unexpected diversity of consumed gastropods in vietnam |
| topic | Edible marine gastropods Museomics Traceability Food safety Alternative protein source Biodiversity |
| url | http://www.sciencedirect.com/science/article/pii/S2666833525001510 |
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