Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis
Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility. Although a huge number of long non-coding RNAs (lncRNAs) are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis, their functions and...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-11-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923002010 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839642851189719040 |
|---|---|
| author | Dan Chu Bin Chen Bo Weng Saina Yan Yanfei Yin Xiangwei Tang Maoliang Ran |
| author_facet | Dan Chu Bin Chen Bo Weng Saina Yan Yanfei Yin Xiangwei Tang Maoliang Ran |
| author_sort | Dan Chu |
| collection | DOAJ |
| description | Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility. Although a huge number of long non-coding RNAs (lncRNAs) are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis, their functions and regulatory mechanisms are still in infancy. Herein, we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells. The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells. lncFPFSC overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis, whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects. Mechanistically, lncFPFSC acted as a sponge for miR-326. Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis, which further abolished the effects of lncFPFSC overexpression. The euchromatic histone-lysine N-methyltransferase 2 (EHMT2) gene was directly targeted by miR-326, and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells. Then, siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition. Collectively, lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis. This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells, and the competing endogenous RNA (ceRNA) network, and provided new molecular markers to treat Sertoli cell disorder inducing male infertility. |
| format | Article |
| id | doaj-art-31e95f5f213e44c58acd9d4f858c8c29 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-31e95f5f213e44c58acd9d4f858c8c292025-07-02T05:39:44ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192024-11-01231138303842Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axisDan Chu0Bin Chen1Bo Weng2Saina Yan3Yanfei Yin4Xiangwei Tang5Maoliang Ran6Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Correspondence Bin ChenHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Xiangxi Vocational and Technical College for Nationalities, Xiangxi 416007, ChinaHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaHunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Correspondence Maoliang RanSertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility. Although a huge number of long non-coding RNAs (lncRNAs) are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis, their functions and regulatory mechanisms are still in infancy. Herein, we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells. The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells. lncFPFSC overexpression promoted cell cycle progression and cell proliferation, as well as inhibited cell apoptosis, whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects. Mechanistically, lncFPFSC acted as a sponge for miR-326. Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis, which further abolished the effects of lncFPFSC overexpression. The euchromatic histone-lysine N-methyltransferase 2 (EHMT2) gene was directly targeted by miR-326, and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells. Then, siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition. Collectively, lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis. This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells, and the competing endogenous RNA (ceRNA) network, and provided new molecular markers to treat Sertoli cell disorder inducing male infertility.http://www.sciencedirect.com/science/article/pii/S2095311923002010porcine Sertoli cellsproliferationapoptosislncRNA FPFSCmiR-326EHMT2 gene |
| spellingShingle | Dan Chu Bin Chen Bo Weng Saina Yan Yanfei Yin Xiangwei Tang Maoliang Ran Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis Journal of Integrative Agriculture porcine Sertoli cells proliferation apoptosis lncRNA FPFSC miR-326 EHMT2 gene |
| title | Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis |
| title_full | Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis |
| title_fullStr | Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis |
| title_full_unstemmed | Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis |
| title_short | Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis |
| title_sort | long non coding rna fpfsc promotes immature porcine sertoli cell growth through modulating the mir 326 ehmt2 axis |
| topic | porcine Sertoli cells proliferation apoptosis lncRNA FPFSC miR-326 EHMT2 gene |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923002010 |
| work_keys_str_mv | AT danchu longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT binchen longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT boweng longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT sainayan longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT yanfeiyin longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT xiangweitang longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis AT maoliangran longnoncodingrnafpfscpromotesimmatureporcinesertolicellgrowththroughmodulatingthemir326ehmt2axis |