Binding site of toxic protein from Arthrinium phaeospermum on plasmalemma of hybrid bamboo
To explore the binding site of toxic protein from Arthrinium phaeospermum on plasmalemma of hybrid bamboo and the binding activities of different hybrid bamboo varieties, based on the separation and purification of toxic protein by a low-pressure chromatography, the specific antiserum against the to...
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| Main Authors: | , |
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| Format: | Article |
| Sprog: | engelsk |
| Udgivet: |
Zhejiang University Press
2012-07-01
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| Serier: | 浙江大学学报. 农业与生命科学版 |
| Fag: | |
| Online adgang: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.04.001 |
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| Summary: | To explore the binding site of toxic protein from Arthrinium phaeospermum on plasmalemma of hybrid bamboo and the binding activities of different hybrid bamboo varieties, based on the separation and purification of toxic protein by a low-pressure chromatography, the specific antiserum against the toxin was prepared by injecting the New Zealand white rabbit with the purified toxin. The results showed that the symptom was alleviated to different degree when the toxin absorbed by different concentration antibodies was inoculated the hybrid bamboo, illuminating that the prepared antibody not only immunologically reacted with the toxin, but also partly blocked some sites of toxin molecular which could recognize the binding sites on the hybrid bamboo cells. The binding activity of plasmalemma preparation of tender branches with toxic protein was determined by a competitive enzyme-linked immunosorbent assay (ELISA). The results revealed that the immunological reaction of the toxin and the antibody could be inhibited by the plasmalemma preparation, which hinted that the plasmalemma preparation contained the binding sites of the toxin. But the binding activities of different varieties had different. After treated by trypsin or heating, the plasmalemma preparation could not inhibit the immunological reaction of the toxin and the antibody, verifying that the protein in the plasmalemma preparation was responsible for binding with the toxin. |
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| ISSN: | 1008-9209 2097-5155 |