INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE
This study presents data upon the effects of CpG oligonucleotide substance (CpG ODN 2006). The commercial preparation was applied in vitro as a single agent, or in combination with various cytokine production inducers. Spontaneous and induced cytokine levels were tested in cultures of human blood ce...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2015-01-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/765 |
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| author | S. N. Klyueva T. P. Shmelkova T. N. Schukovskaya |
| author_facet | S. N. Klyueva T. P. Shmelkova T. N. Schukovskaya |
| author_sort | S. N. Klyueva |
| collection | DOAJ |
| description | This study presents data upon the effects of CpG oligonucleotide substance (CpG ODN 2006). The commercial preparation was applied in vitro as a single agent, or in combination with various cytokine production inducers. Spontaneous and induced cytokine levels were tested in cultures of human blood cells taken from the persons before and after anti-plague vaccination. It has been shown that the vaccination had no effect upon spontaneous production of cytokines participating in innate (IL-8, TNFα) and adaptive (IFNγ, IL-4) immunity. CpG ODN 2006 proved to exert effects upon functional activity of human blood Th1 and Th2 pro-inflammatory cytokines after the first vaccination with live anti-plague vaccine, being reflected as higher Th1/ proinflammatory (IL-17 and IFNг) and Th2/ antiinflammatory (IL-4) cytokine production. Postvaccination changes of IgM, IgG, IgA, and IgE contents were non-significant. Following repeated vaccination, the CpG ODN 2006 induced an increase of IL-4 and IL-17 production in blood cells, as well as decrease in IFNг levels. Thus, the ability of CpG ODN 2006 to activate the cells participating in innate and adaptive immunity opens some prospects for usage of this oligodeoxynucleotide in the development of more efficient anti-plague vaccines. |
| format | Article |
| id | doaj-art-2f70e24f59f3496e92f5e4888e657b67 |
| institution | Matheson Library |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2015-01-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-2f70e24f59f3496e92f5e4888e657b672025-08-04T14:30:33ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2015-01-0116653153810.15789/1563-0625-2014-6-531-538719INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUES. N. Klyueva0T. P. Shmelkova1T. N. Schukovskaya2Russian Research Anti-Plague Institute “Microbe”, Saratov, Russian FederationRussian Research Anti-Plague Institute “Microbe”, Saratov, Russian FederationRussian Research Anti-Plague Institute “Microbe”, Saratov, Russian FederationThis study presents data upon the effects of CpG oligonucleotide substance (CpG ODN 2006). The commercial preparation was applied in vitro as a single agent, or in combination with various cytokine production inducers. Spontaneous and induced cytokine levels were tested in cultures of human blood cells taken from the persons before and after anti-plague vaccination. It has been shown that the vaccination had no effect upon spontaneous production of cytokines participating in innate (IL-8, TNFα) and adaptive (IFNγ, IL-4) immunity. CpG ODN 2006 proved to exert effects upon functional activity of human blood Th1 and Th2 pro-inflammatory cytokines after the first vaccination with live anti-plague vaccine, being reflected as higher Th1/ proinflammatory (IL-17 and IFNг) and Th2/ antiinflammatory (IL-4) cytokine production. Postvaccination changes of IgM, IgG, IgA, and IgE contents were non-significant. Following repeated vaccination, the CpG ODN 2006 induced an increase of IL-4 and IL-17 production in blood cells, as well as decrease in IFNг levels. Thus, the ability of CpG ODN 2006 to activate the cells participating in innate and adaptive immunity opens some prospects for usage of this oligodeoxynucleotide in the development of more efficient anti-plague vaccines.https://www.mimmun.ru/mimmun/article/view/765vaccineoligodeoxynucleotide cpg odn 2006cytokine production |
| spellingShingle | S. N. Klyueva T. P. Shmelkova T. N. Schukovskaya INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE Медицинская иммунология vaccine oligodeoxynucleotide cpg odn 2006 cytokine production |
| title | INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE |
| title_full | INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE |
| title_fullStr | INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE |
| title_full_unstemmed | INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE |
| title_short | INFLUENCE OF CpG ODN 2006 OLIGODEOXYNUCLEOTIDE ON CYTOKINE PRODUCTION BY BLOOD CELLS OF HUMANS VACCINATED AGAINST PLAGUE |
| title_sort | influence of cpg odn 2006 oligodeoxynucleotide on cytokine production by blood cells of humans vaccinated against plague |
| topic | vaccine oligodeoxynucleotide cpg odn 2006 cytokine production |
| url | https://www.mimmun.ru/mimmun/article/view/765 |
| work_keys_str_mv | AT snklyueva influenceofcpgodn2006oligodeoxynucleotideoncytokineproductionbybloodcellsofhumansvaccinatedagainstplague AT tpshmelkova influenceofcpgodn2006oligodeoxynucleotideoncytokineproductionbybloodcellsofhumansvaccinatedagainstplague AT tnschukovskaya influenceofcpgodn2006oligodeoxynucleotideoncytokineproductionbybloodcellsofhumansvaccinatedagainstplague |