Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma
The role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-11-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317717140 |
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| author | Kamil Zalewski Marcin Misiek Artur Kowalik Elwira Bakuła-Zalewska Janusz Kopczyński Aleksandra Zielińska Mariusz Bidziński Jakub Radziszewski Stanisław Góźdź Magdalena Kowalewska |
| author_facet | Kamil Zalewski Marcin Misiek Artur Kowalik Elwira Bakuła-Zalewska Janusz Kopczyński Aleksandra Zielińska Mariusz Bidziński Jakub Radziszewski Stanisław Góźdź Magdalena Kowalewska |
| author_sort | Kamil Zalewski |
| collection | DOAJ |
| description | The role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar intraepithelial neoplasia lesions and vulvar squamous cell carcinoma. The purpose of this study was to select appropriate references for gene expression studies in plasma of patients with these lesions. Expression levels of six microRNAs—hsa-miR-425-5p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-5p, hsa-miR-103a-3p, and hsa-miR-16-5p—were analyzed by quantitative reverse transcription polymerase chain reaction in plasma samples obtained from 17 patients with vulvar intraepithelial neoplasia lesion and 27 patients with vulvar squamous cell carcinoma. The expression stability of these candidate normalizers was assayed using geNorm algorithm. hsa-miR-93-5p was revealed as the most stably expressed reference in plasma samples of both vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients. The results pointed at hsa-miR-93-5p and hsa-miR-425-5p as microRNAs that retained the greatest robustness in plasma of vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients, respectively. Our work is the first report on reference microRNA selection for quantitative real-time polymerase chain reaction applications in vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma. The candidate microRNA stability values for the two types of lesions are provided and might serve for normalization of the future novel microRNA biomarkers in these rare entities. |
| format | Article |
| id | doaj-art-2eaeab66a34a46c19ece6d78e4e217d1 |
| institution | Matheson Library |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-2eaeab66a34a46c19ece6d78e4e217d12025-07-02T05:19:01ZengSAGE PublishingTumor Biology1423-03802017-11-013910.1177/1010428317717140Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinomaKamil Zalewski0Marcin Misiek1Artur Kowalik2Elwira Bakuła-Zalewska3Janusz Kopczyński4Aleksandra Zielińska5Mariusz Bidziński6Jakub Radziszewski7Stanisław Góźdź8Magdalena Kowalewska9Department of Molecular and Translational Oncology, Maria Skłodowska-Curie Institute—Oncology Center, Warsaw, PolandDepartment of Gynecologic Oncology, Holycross Cancer Center, Kielce, PolandDepartment Molecular Diagnostics, Holycross Cancer Center, Kielce, PolandDepartment of Pathology, Maria Skłodowska-Curie Institute—Oncology Center, Warsaw, PolandDepartment of Pathology, Holycross Cancer Center, Kielce, PolandDepartment of Obstetrics, Gynecology and Oncology, 2nd Faculty of Medicine, Warsaw Medical University, Warsaw, PolandDepartment of Gynecologic Oncology, Maria Skłodowska-Curie Institute—Oncology Center, Warsaw, PolandDepartment of General Vascular and Oncological Surgery, Multidisciplinary Hospital Warsaw-Międzylesie, PolandDepartment of Clinical Oncology, Holycross Cancer Center, Kielce, PolandDepartment of Immunology, Biochemistry and Nutrition, Medical University of Warsaw, Warsaw, PolandThe role of circulating microRNAs as a promising tool for diagnosing cancer and monitoring anticancer therapies has been widely studied in the past decades. To date, no suitable reference microRNAs for normalizing quantitative real-time polymerase chain reaction assays has been identified in vulvar intraepithelial neoplasia lesions and vulvar squamous cell carcinoma. The purpose of this study was to select appropriate references for gene expression studies in plasma of patients with these lesions. Expression levels of six microRNAs—hsa-miR-425-5p, hsa-miR-191-5p, hsa-miR-93-5p, hsa-miR-423-5p, hsa-miR-103a-3p, and hsa-miR-16-5p—were analyzed by quantitative reverse transcription polymerase chain reaction in plasma samples obtained from 17 patients with vulvar intraepithelial neoplasia lesion and 27 patients with vulvar squamous cell carcinoma. The expression stability of these candidate normalizers was assayed using geNorm algorithm. hsa-miR-93-5p was revealed as the most stably expressed reference in plasma samples of both vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients. The results pointed at hsa-miR-93-5p and hsa-miR-425-5p as microRNAs that retained the greatest robustness in plasma of vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma patients, respectively. Our work is the first report on reference microRNA selection for quantitative real-time polymerase chain reaction applications in vulvar intraepithelial neoplasia lesion and vulvar squamous cell carcinoma. The candidate microRNA stability values for the two types of lesions are provided and might serve for normalization of the future novel microRNA biomarkers in these rare entities.https://doi.org/10.1177/1010428317717140 |
| spellingShingle | Kamil Zalewski Marcin Misiek Artur Kowalik Elwira Bakuła-Zalewska Janusz Kopczyński Aleksandra Zielińska Mariusz Bidziński Jakub Radziszewski Stanisław Góźdź Magdalena Kowalewska Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma Tumor Biology |
| title | Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| title_full | Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| title_fullStr | Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| title_full_unstemmed | Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| title_short | Normalizers for microRNA quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| title_sort | normalizers for microrna quantification in plasma of patients with vulvar intraepithelial neoplasia lesions and vulvar carcinoma |
| url | https://doi.org/10.1177/1010428317717140 |
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