DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD
A new method for determination of moxifloxacin in human plasma using HPLC with UV-detection is described. The sample preparation was made by protein precipitation by 50% trifluoracetic acid solution. The method was validated in terms of selectivity, calibration curve, accuracy, precision, lower limi...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2019-01-01
|
| Series: | Разработка и регистрация лекарственных средств |
| Subjects: | |
| Online Access: | https://www.pharmjournal.ru/jour/article/view/307 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839586772672053248 |
|---|---|
| author | T. N. Komarov Yu. V. Medvedev I. E. Shohin Yu. E. Boldina A. A. Lvova E. S. Melnikov E. N. Fisher R. V. Ivanov R. J. Maksvitis |
| author_facet | T. N. Komarov Yu. V. Medvedev I. E. Shohin Yu. E. Boldina A. A. Lvova E. S. Melnikov E. N. Fisher R. V. Ivanov R. J. Maksvitis |
| author_sort | T. N. Komarov |
| collection | DOAJ |
| description | A new method for determination of moxifloxacin in human plasma using HPLC with UV-detection is described. The sample preparation was made by protein precipitation by 50% trifluoracetic acid solution. The method was validated in terms of selectivity, calibration curve, accuracy, precision, lower limit of quantification, carry-over and stability. The analytical range was 100-5000 ng/mL. Limit of determination was 31 ng/mL. Method could be applied to moxifloxacin determination in plasma for PK and BE studies. |
| format | Article |
| id | doaj-art-2c12f4c9ec2e4e6daa389810a5935d3a |
| institution | Matheson Library |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| publishDate | 2019-01-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj-art-2c12f4c9ec2e4e6daa389810a5935d3a2025-08-03T19:23:53ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492019-01-0103174179307DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHODT. N. Komarov0Yu. V. Medvedev1I. E. Shohin2Yu. E. Boldina3A. A. Lvova4E. S. Melnikov5E. N. Fisher6R. V. Ivanov7R. J. Maksvitis8Center of Pharmaceutical Analytics Ltd.Center of Pharmaceutical Analytics Ltd.; I.M. Sechenov First Moscow State Medical UniversityCenter of Pharmaceutical Analytics Ltd.Center of Pharmaceutical Analytics Ltd.Center of Pharmaceutical Analytics Ltd.Center of Pharmaceutical Analytics Ltd.Center of Pharmaceutical Analytics Ltd.; I.M. Sechenov First Moscow State Medical UniversityR&D PHARMA Ltd.R&D PHARMA Ltd.A new method for determination of moxifloxacin in human plasma using HPLC with UV-detection is described. The sample preparation was made by protein precipitation by 50% trifluoracetic acid solution. The method was validated in terms of selectivity, calibration curve, accuracy, precision, lower limit of quantification, carry-over and stability. The analytical range was 100-5000 ng/mL. Limit of determination was 31 ng/mL. Method could be applied to moxifloxacin determination in plasma for PK and BE studies.https://www.pharmjournal.ru/jour/article/view/307moxifloxacinplasmahplc-uv |
| spellingShingle | T. N. Komarov Yu. V. Medvedev I. E. Shohin Yu. E. Boldina A. A. Lvova E. S. Melnikov E. N. Fisher R. V. Ivanov R. J. Maksvitis DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD Разработка и регистрация лекарственных средств moxifloxacin plasma hplc-uv |
| title | DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD |
| title_full | DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD |
| title_fullStr | DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD |
| title_short | DEVELOPMENT AND VALIDATION OF MOXIFLOXACIN DETERMINATION IN HUMAN PLASMA BY HPLC-UV METHOD |
| title_sort | development and validation of moxifloxacin determination in human plasma by hplc uv method |
| topic | moxifloxacin plasma hplc-uv |
| url | https://www.pharmjournal.ru/jour/article/view/307 |
| work_keys_str_mv | AT tnkomarov developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT yuvmedvedev developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT ieshohin developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT yueboldina developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT aalvova developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT esmelnikov developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT enfisher developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT rvivanov developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod AT rjmaksvitis developmentandvalidationofmoxifloxacindeterminationinhumanplasmabyhplcuvmethod |