Production and characterization of monoclonal antibody to recombinant chicken IL-2
Four hybridoma cell lines named as 1A12, 4A3, 3E7 and 4E1, secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.05.0541 |
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| Summary: | Four hybridoma cell lines named as 1A12, 4A3, 3E7 and 4E1, secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows: The ELISA titers were 5.12× 10<sup>5</sup>, 1.02×10<sup>6</sup>, 2.56×10<sup>5</sup> and 4.09×10<sup>6</sup>, respectively. All the Mabs failed into type IgG1. Western-blot analysis showed that the four MAbs could react with chicken IL-2. |
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| ISSN: | 1008-9209 2097-5155 |