Overcoming challenges in metagenomic AMR surveillance with nanopore sequencing: a case study on fluoroquinolone resistance

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Overcoming challenges in metagenomic AMR surveillance with nanopore sequencing: a case study on fluoroquinolone resistance

IntroductionAntimicrobial resistance is an alarming public health problem, and comprehensive surveillance across environments is required to reduce its impact. Phenotypic testing and whole-genome sequencing of isolates are efficient, but culture-free approaches like metagenomic sequencing potentiall...

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Main Authors:	Bram Bloemen, Mathieu Gand, Moniek Ringenier, Bert Bogaerts, Kevin Vanneste, Kathleen Marchal, Nancy H. C. Roosens, Jeroen Dewulf, Filip Boyen, Sigrid C. J. De Keersmaecker
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-07-01
Series:	Frontiers in Microbiology
Subjects:	metagenomic sequencing antimicrobial resistance (AMR) nanopore sequencing DNA methylation strain-resolved metagenomics plasmid host prediction
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2025.1614301/full
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