Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations
Corticosterone (CCS) concentration in chicken skeletal muscle could be a potential Animal-Based Measure that could give ex-post indications of adaptive responses to different farming systems and management practices. The aim of the present trial was to measure CCS concentrations in chicken skeletal...
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Elsevier
2025-09-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125007199 |
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| author | Luca Todini Laura Menchetti Roberta Stocchi David Ranucci Olimpia Barbato Giovanni Ricci |
| author_facet | Luca Todini Laura Menchetti Roberta Stocchi David Ranucci Olimpia Barbato Giovanni Ricci |
| author_sort | Luca Todini |
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| description | Corticosterone (CCS) concentration in chicken skeletal muscle could be a potential Animal-Based Measure that could give ex-post indications of adaptive responses to different farming systems and management practices. The aim of the present trial was to measure CCS concentrations in chicken skeletal muscle and assess their relationship with total plasma CCS concentrations. Blood and muscle samples were recovered respectively at the beginning and the end of an industrial slaughter line from 48 identified female broilers aged 84 d. Muscle juice samples were obtained by high-speed centrifugation, and analytical validation of a commercial CCS EIA kit has been carried out. Regarding repeatability (precision), intra-assay coefficient of variation (CV, n = 12 replicates) resulted 4.2 %. Inter-assay CV (2 replicates in n = 4 assay sessions) was 11.9 %. The line resulting from serial dilutions of a pooled muscle juice sample has been compared with the calibrators line for parallelism and with the expected values line for linearity. The slopes of the obtained and calibrators lines were not different (F = 4.082, P = 0.090). A strong correlation was found between the observed and expected concentrations (Pearson correlation coefficient, r = 0.991, P < 0.001). The mean RR (recovery rate, accuracy) of different quantities of hormone added to the pooled muscle juice was 90.0 ± 1.6 %. The mean CCS concentration in muscle juice samples was 455.1 ± 46.8 pg/mL (range from 47.5 to 1,340.0 pg/mL). The mean total plasma CCS concentration was 11.2 ± 0.4 ng/mL (range 7.0 to 19.5 ng/mL). The mean ratio of individual muscle:plasma CCS concentration was 0.040 ± 0.004, ranging from 0.005 to 0.133. Muscle and plasma CCS levels were moderately correlated (r = 0.324, P = 0.025). It is concluded that this EIA kit can provide reliable results for measuring CCS concentrations in juice obtained by high-speed centrifugation of chicken muscle, directly without previous extraction. Such a method can be a further tool for the assessment of animal physiological responses, stress, and welfare. |
| format | Article |
| id | doaj-art-2b57c08d8f9e490aa08db77c3fd9f2a0 |
| institution | Matheson Library |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-2b57c08d8f9e490aa08db77c3fd9f2a02025-06-30T04:08:43ZengElsevierPoultry Science0032-57912025-09-011049105475Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrationsLuca Todini0Laura Menchetti1Roberta Stocchi2David Ranucci3Olimpia Barbato4Giovanni Ricci5School of Biosciences and Veterinary Medicine, University of Camerino, Via della Circonvallazione 93/95, 62024 Matelica, MC, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via della Circonvallazione 93/95, 62024 Matelica, MC, Italy; Corresponding author.School of Biosciences and Veterinary Medicine, University of Camerino, Via della Circonvallazione 93/95, 62024 Matelica, MC, ItalyDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, ItalyDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, ItalyDepartment of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, ItalyCorticosterone (CCS) concentration in chicken skeletal muscle could be a potential Animal-Based Measure that could give ex-post indications of adaptive responses to different farming systems and management practices. The aim of the present trial was to measure CCS concentrations in chicken skeletal muscle and assess their relationship with total plasma CCS concentrations. Blood and muscle samples were recovered respectively at the beginning and the end of an industrial slaughter line from 48 identified female broilers aged 84 d. Muscle juice samples were obtained by high-speed centrifugation, and analytical validation of a commercial CCS EIA kit has been carried out. Regarding repeatability (precision), intra-assay coefficient of variation (CV, n = 12 replicates) resulted 4.2 %. Inter-assay CV (2 replicates in n = 4 assay sessions) was 11.9 %. The line resulting from serial dilutions of a pooled muscle juice sample has been compared with the calibrators line for parallelism and with the expected values line for linearity. The slopes of the obtained and calibrators lines were not different (F = 4.082, P = 0.090). A strong correlation was found between the observed and expected concentrations (Pearson correlation coefficient, r = 0.991, P < 0.001). The mean RR (recovery rate, accuracy) of different quantities of hormone added to the pooled muscle juice was 90.0 ± 1.6 %. The mean CCS concentration in muscle juice samples was 455.1 ± 46.8 pg/mL (range from 47.5 to 1,340.0 pg/mL). The mean total plasma CCS concentration was 11.2 ± 0.4 ng/mL (range 7.0 to 19.5 ng/mL). The mean ratio of individual muscle:plasma CCS concentration was 0.040 ± 0.004, ranging from 0.005 to 0.133. Muscle and plasma CCS levels were moderately correlated (r = 0.324, P = 0.025). It is concluded that this EIA kit can provide reliable results for measuring CCS concentrations in juice obtained by high-speed centrifugation of chicken muscle, directly without previous extraction. Such a method can be a further tool for the assessment of animal physiological responses, stress, and welfare.http://www.sciencedirect.com/science/article/pii/S0032579125007199BroilerCorticosteroneMuscleWelfareEnzyme Immunoassay |
| spellingShingle | Luca Todini Laura Menchetti Roberta Stocchi David Ranucci Olimpia Barbato Giovanni Ricci Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations Poultry Science Broiler Corticosterone Muscle Welfare Enzyme Immunoassay |
| title | Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| title_full | Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| title_fullStr | Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| title_full_unstemmed | Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| title_short | Measuring corticosterone concentrations in broiler muscle: Analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| title_sort | measuring corticosterone concentrations in broiler muscle analytical validation of an enzyme immuno assay kit and relationships with total plasma concentrations |
| topic | Broiler Corticosterone Muscle Welfare Enzyme Immunoassay |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125007199 |
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