Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources
The claustrum is thought to be one of the most highly interconnected forebrain structures, but its organizing principles have yet to be fully explored at the level of single neurons. Here, we investigated the identity, connectivity, and activity of identified claustrum neurons in Mus musculus to und...
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eLife Sciences Publications Ltd
2025-07-01
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| Online Access: | https://elifesciences.org/articles/98002 |
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| author | Andrew M Shelton David K Oliver Ivan P Lazarte Joachim S Grimstvedt Ishaan Kapoor Jake A Swann Caitlin A Ashcroft Simon N Williams Niall Conway Selma Tir Amy Robinson Stuart Peirson Thomas Akam Clifford G Kentros Menno P Witter Simon JB Butt Adam Max Packer |
| author_facet | Andrew M Shelton David K Oliver Ivan P Lazarte Joachim S Grimstvedt Ishaan Kapoor Jake A Swann Caitlin A Ashcroft Simon N Williams Niall Conway Selma Tir Amy Robinson Stuart Peirson Thomas Akam Clifford G Kentros Menno P Witter Simon JB Butt Adam Max Packer |
| author_sort | Andrew M Shelton |
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| description | The claustrum is thought to be one of the most highly interconnected forebrain structures, but its organizing principles have yet to be fully explored at the level of single neurons. Here, we investigated the identity, connectivity, and activity of identified claustrum neurons in Mus musculus to understand how the structure’s unique convergence of input and divergence of output support binding information streams. We found that neurons in the claustrum communicate with each other across efferent projection-defined modules which were differentially innervated by sensory and frontal cortical areas. Individual claustrum neurons were responsive to inputs from more than one cortical region in a cell-type and projection-specific manner, particularly between areas of frontal cortex. In vivo imaging of claustrum axons revealed responses to both unimodal and multimodal sensory stimuli. Finally, chronic claustrum silencing specifically reduced animals’ sensitivity to multimodal stimuli. These findings support the view that the claustrum is a fundamentally integrative structure, consolidating information from around the cortex and redistributing it following local computations. |
| format | Article |
| id | doaj-art-2b2cb1b6affb45c8be7354525a48fc30 |
| institution | Matheson Library |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj-art-2b2cb1b6affb45c8be7354525a48fc302025-07-16T11:59:16ZengeLife Sciences Publications LtdeLife2050-084X2025-07-011310.7554/eLife.98002Single neurons and networks in the mouse claustrum integrate input from widespread cortical sourcesAndrew M Shelton0https://orcid.org/0000-0002-5787-4310David K Oliver1https://orcid.org/0000-0003-1210-8409Ivan P Lazarte2Joachim S Grimstvedt3Ishaan Kapoor4Jake A Swann5Caitlin A Ashcroft6Simon N Williams7Niall Conway8Selma Tir9Amy Robinson10Stuart Peirson11Thomas Akam12https://orcid.org/0000-0002-1810-0494Clifford G Kentros13Menno P Witter14https://orcid.org/0000-0003-0285-1637Simon JB Butt15https://orcid.org/0000-0002-2399-0102Adam Max Packer16https://orcid.org/0000-0001-5884-794XDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomKavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, CanadaDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomNuffield Department of Clinical Neurosciences, Sir Jules Thorn Sleep and Circadian Neuroscience Institute (SCNi), University of Oxford, Oxford, United KingdomKavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, CanadaNuffield Department of Clinical Neurosciences, Sir Jules Thorn Sleep and Circadian Neuroscience Institute (SCNi), University of Oxford, Oxford, United KingdomDepartment of Experimental Psychology, University of Oxford, Oxford, United KingdomKavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, CanadaKavli Institute for Systems Neuroscience, Centre for Neural Computation, Norwegian University of Science and Technology, Trondheim, CanadaDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomDepartment of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United KingdomThe claustrum is thought to be one of the most highly interconnected forebrain structures, but its organizing principles have yet to be fully explored at the level of single neurons. Here, we investigated the identity, connectivity, and activity of identified claustrum neurons in Mus musculus to understand how the structure’s unique convergence of input and divergence of output support binding information streams. We found that neurons in the claustrum communicate with each other across efferent projection-defined modules which were differentially innervated by sensory and frontal cortical areas. Individual claustrum neurons were responsive to inputs from more than one cortical region in a cell-type and projection-specific manner, particularly between areas of frontal cortex. In vivo imaging of claustrum axons revealed responses to both unimodal and multimodal sensory stimuli. Finally, chronic claustrum silencing specifically reduced animals’ sensitivity to multimodal stimuli. These findings support the view that the claustrum is a fundamentally integrative structure, consolidating information from around the cortex and redistributing it following local computations.https://elifesciences.org/articles/98002claustrumcortexconnectivity |
| spellingShingle | Andrew M Shelton David K Oliver Ivan P Lazarte Joachim S Grimstvedt Ishaan Kapoor Jake A Swann Caitlin A Ashcroft Simon N Williams Niall Conway Selma Tir Amy Robinson Stuart Peirson Thomas Akam Clifford G Kentros Menno P Witter Simon JB Butt Adam Max Packer Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources eLife claustrum cortex connectivity |
| title | Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| title_full | Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| title_fullStr | Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| title_full_unstemmed | Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| title_short | Single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| title_sort | single neurons and networks in the mouse claustrum integrate input from widespread cortical sources |
| topic | claustrum cortex connectivity |
| url | https://elifesciences.org/articles/98002 |
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