Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types
ABSTRACT: Staphylococcus aureus intramammary infections often leads to clinical and subclinical mastitis in dairy cattle. Prediction of disease evolution and treatment efficacy based on the characteristics of disease-causing strains of S. aureus would significantly improve management of dairy herds....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224010610 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839617162819403776 |
|---|---|
| author | E. Demontier C. Ster S. Chamberland S. Ramanathan S. Dufour F. Malouin |
| author_facet | E. Demontier C. Ster S. Chamberland S. Ramanathan S. Dufour F. Malouin |
| author_sort | E. Demontier |
| collection | DOAJ |
| description | ABSTRACT: Staphylococcus aureus intramammary infections often leads to clinical and subclinical mastitis in dairy cattle. Prediction of disease evolution and treatment efficacy based on the characteristics of disease-causing strains of S. aureus would significantly improve management of dairy herds. To study the effect of biofilm production and the influence of genetic lineage, we selected S. aureus isolates from the most prevalent Canadian spa types associated with bovine mastitis. Antimicrobial susceptibility in planktonic growth and for bacteria embedded in biofilm was compared. PCR was used to detect the bap gene responsible for atypical biofilm formation. All Canadian spa types from dairy cattle were susceptible to the 8 antimicrobial agents tested. Only strain sa3493 from spa type t267 showed a resistance to pirlimycin. However, bacteria producing larger amounts of biofilms better survived the bactericidal action of antimicrobial agents even when exposed to concentrations 64 folds higher than the minimal inhibitory concentration determined for planktonic cultures. Pirlimycin was more effective on bacteria producing low to moderate levels of biofilm compared with vancomycin or ceftiofur. Antimicrobial agents did not affect the viability of spa types t13401 and t605 that were high biofilm producers. Although both these spa types produced high amounts of biofilm, only t605 possessed the bap gene. We also found a close relationship between DIM at sampling and the presence of spa type t605 isolates. These results suggest that detection of S. aureus spa type may help predict the effectiveness of antimicrobial therapy and that some spa types are more likely to be retrieved toward the end of the lactation. |
| format | Article |
| id | doaj-art-2a7cf1b0c059432bba5955b00a4037b8 |
| institution | Matheson Library |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-2a7cf1b0c059432bba5955b00a4037b82025-07-25T04:17:53ZengElsevierJournal of Dairy Science0022-03022025-08-01108881768186Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa typesE. Demontier0C. Ster1S. Chamberland2S. Ramanathan3S. Dufour4F. Malouin5Faculté des sciences, Département de biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada; Regroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, CanadaFaculté des sciences, Département de biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada; Regroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, Canada; Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, QC J1M 0C8, CanadaFaculté des sciences, Département de biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada; Regroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, CanadaRegroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, Canada; Département d'immunologie et de biologie cellulaire, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada,Regroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, Canada; Département de pathologie et de microbiologie, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, QC J2S 2M2, Canada,Faculté des sciences, Département de biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada; Regroupement stratégique FRQNT pour un lait de qualité optimale, Op+lait, Université de Montréal, St-Hyacinthe, QC J2S 2M2, Canada; Corresponding authorABSTRACT: Staphylococcus aureus intramammary infections often leads to clinical and subclinical mastitis in dairy cattle. Prediction of disease evolution and treatment efficacy based on the characteristics of disease-causing strains of S. aureus would significantly improve management of dairy herds. To study the effect of biofilm production and the influence of genetic lineage, we selected S. aureus isolates from the most prevalent Canadian spa types associated with bovine mastitis. Antimicrobial susceptibility in planktonic growth and for bacteria embedded in biofilm was compared. PCR was used to detect the bap gene responsible for atypical biofilm formation. All Canadian spa types from dairy cattle were susceptible to the 8 antimicrobial agents tested. Only strain sa3493 from spa type t267 showed a resistance to pirlimycin. However, bacteria producing larger amounts of biofilms better survived the bactericidal action of antimicrobial agents even when exposed to concentrations 64 folds higher than the minimal inhibitory concentration determined for planktonic cultures. Pirlimycin was more effective on bacteria producing low to moderate levels of biofilm compared with vancomycin or ceftiofur. Antimicrobial agents did not affect the viability of spa types t13401 and t605 that were high biofilm producers. Although both these spa types produced high amounts of biofilm, only t605 possessed the bap gene. We also found a close relationship between DIM at sampling and the presence of spa type t605 isolates. These results suggest that detection of S. aureus spa type may help predict the effectiveness of antimicrobial therapy and that some spa types are more likely to be retrieved toward the end of the lactation.http://www.sciencedirect.com/science/article/pii/S0022030224010610Staphylococcus aureusantimicrobial susceptibilitybiofilmmastitisdays in milk |
| spellingShingle | E. Demontier C. Ster S. Chamberland S. Ramanathan S. Dufour F. Malouin Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types Journal of Dairy Science Staphylococcus aureus antimicrobial susceptibility biofilm mastitis days in milk |
| title | Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types |
| title_full | Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types |
| title_fullStr | Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types |
| title_full_unstemmed | Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types |
| title_short | Biofilm Formation in Dairy: A Food Safety Concern—Effect of biofilm production on antimicrobial susceptibility of Staphylococcus aureus bovine mastitis strains from the most prevalent Canadian spa types |
| title_sort | biofilm formation in dairy a food safety concern effect of biofilm production on antimicrobial susceptibility of staphylococcus aureus bovine mastitis strains from the most prevalent canadian spa types |
| topic | Staphylococcus aureus antimicrobial susceptibility biofilm mastitis days in milk |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224010610 |
| work_keys_str_mv | AT edemontier biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes AT cster biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes AT schamberland biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes AT sramanathan biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes AT sdufour biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes AT fmalouin biofilmformationindairyafoodsafetyconcerneffectofbiofilmproductiononantimicrobialsusceptibilityofstaphylococcusaureusbovinemastitisstrainsfromthemostprevalentcanadianspatypes |