Expression of chicken interleukin-2 gene in Pichia methanolica
Chicken interleukin-2 (chIL-2) is an important cytokine discovered recently. To explore a new approach to the expression of chIL-2 gene in methyltrophic yeast-Pichia methanolica (P. methanolica), the chIL-2 gene was obtained by PCR and inserted into the expression vector pMETαA. The recombinant P. m...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.04.0419 |
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| Summary: | Chicken interleukin-2 (chIL-2) is an important cytokine discovered recently. To explore a new approach to the expression of chIL-2 gene in methyltrophic yeast-Pichia methanolica (P. methanolica), the chIL-2 gene was obtained by PCR and inserted into the expression vector pMETαA. The recombinant P. methanolica PMAD11 strain (pMETαA/rchIL-2) was constructed using homologues recombination. SDS-PAGE and Western-blot analyses showed that the rchIL-2 protein was secreted successfully in the culture medium by P. methanolica PMAD11 strain, which have a molecular weight of 16 kDa, amounting to 35 percent of the total secreted proteins. |
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| ISSN: | 1008-9209 2097-5155 |