The variation of NAD+-SDH gene in mutant white-fleshed loquat
Loquat (Eriobotrya japonica Lindl.) can be divided into yellow- and white-fleshed cultivars by flesh color. However, a Dongting loquat mutant, which involved bud sport and growing white-fleshed fruit in the central region of the trunk (as wild loquat bears yellow-fleshed fruits naturally), was disco...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612977 |
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| author | Jing LI Yong-qing WANG Dong CHEN Mei-yan TU Hong-jiang XIE Guo-liang JIANG Jia LIU Shu-xia SUN |
| author_facet | Jing LI Yong-qing WANG Dong CHEN Mei-yan TU Hong-jiang XIE Guo-liang JIANG Jia LIU Shu-xia SUN |
| author_sort | Jing LI |
| collection | DOAJ |
| description | Loquat (Eriobotrya japonica Lindl.) can be divided into yellow- and white-fleshed cultivars by flesh color. However, a Dongting loquat mutant, which involved bud sport and growing white-fleshed fruit in the central region of the trunk (as wild loquat bears yellow-fleshed fruits naturally), was discovered in the preliminary study. The study cloned the coding sequence (CDS) of NAD+-dependent sorbitol dehydrogenase (NAD+-SDH) gene from the selected materials of mutant loquat, wild loquat and other nine loquat cultivars/accessions, and found that the CDS of NAD+-SDH gene from the mutant loquat, other than the rest two types of materials, had three single nucleotide polymorphisms (SNPs) loci; in addition, the amino acid encoded at variation loci changed accordingly. NAD+-SDH plays an active role in converting sorbitol into fructose in loquat cultivars. For the mutant white-fleshed loquat, the activity of NAD+-SDH rises first and then drops, the sorbitol content decreases steadily, and its fructose content is higher than that in wild loquat from coloration to maturation stage. As demonstrated by the real-time fluorescence quantification PCR analysis, the expression level of NAD+-SDH gene at maturation stage is about 5-fold lower than wild type. It may be assumed that, the three SNPs loci might lead to excessive conversion of sorbitol into fructose under the catalytic action of NAD+-SDH of white-fleshed mutant loquat at maturation stage, resulting in the increase of fructose content and reduced expression abundance of mRNA after transcription. Besides, NAD+-SDH gene may be related to flesh color and carbohydrate variation of white-fleshed mutant loquat. |
| format | Article |
| id | doaj-art-2a1f4b4bf7054035be121fd6aadb1b5c |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-2a1f4b4bf7054035be121fd6aadb1b5c2025-07-02T04:50:54ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-08-0115817441750The variation of NAD+-SDH gene in mutant white-fleshed loquatJing LI0Yong-qing WANG1Dong CHEN2Mei-yan TU3Hong-jiang XIE4Guo-liang JIANG5Jia LIU6Shu-xia SUN7College of Horticulture, Sichuan Agricultural University, Ya'an 611130, P.R. China; Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaCollege of Horticulture, Sichuan Agricultural University, Ya'an 611130, P.R. China; WANG Yong-qingHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. ChinaHorticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R. China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu 610066, P.R. China; Correspondence SUN Shu-xiaLoquat (Eriobotrya japonica Lindl.) can be divided into yellow- and white-fleshed cultivars by flesh color. However, a Dongting loquat mutant, which involved bud sport and growing white-fleshed fruit in the central region of the trunk (as wild loquat bears yellow-fleshed fruits naturally), was discovered in the preliminary study. The study cloned the coding sequence (CDS) of NAD+-dependent sorbitol dehydrogenase (NAD+-SDH) gene from the selected materials of mutant loquat, wild loquat and other nine loquat cultivars/accessions, and found that the CDS of NAD+-SDH gene from the mutant loquat, other than the rest two types of materials, had three single nucleotide polymorphisms (SNPs) loci; in addition, the amino acid encoded at variation loci changed accordingly. NAD+-SDH plays an active role in converting sorbitol into fructose in loquat cultivars. For the mutant white-fleshed loquat, the activity of NAD+-SDH rises first and then drops, the sorbitol content decreases steadily, and its fructose content is higher than that in wild loquat from coloration to maturation stage. As demonstrated by the real-time fluorescence quantification PCR analysis, the expression level of NAD+-SDH gene at maturation stage is about 5-fold lower than wild type. It may be assumed that, the three SNPs loci might lead to excessive conversion of sorbitol into fructose under the catalytic action of NAD+-SDH of white-fleshed mutant loquat at maturation stage, resulting in the increase of fructose content and reduced expression abundance of mRNA after transcription. Besides, NAD+-SDH gene may be related to flesh color and carbohydrate variation of white-fleshed mutant loquat.http://www.sciencedirect.com/science/article/pii/S2095311915612977loquatflesh colorNAD+-SDHSNPfructose |
| spellingShingle | Jing LI Yong-qing WANG Dong CHEN Mei-yan TU Hong-jiang XIE Guo-liang JIANG Jia LIU Shu-xia SUN The variation of NAD+-SDH gene in mutant white-fleshed loquat Journal of Integrative Agriculture loquat flesh color NAD+-SDH SNP fructose |
| title | The variation of NAD+-SDH gene in mutant white-fleshed loquat |
| title_full | The variation of NAD+-SDH gene in mutant white-fleshed loquat |
| title_fullStr | The variation of NAD+-SDH gene in mutant white-fleshed loquat |
| title_full_unstemmed | The variation of NAD+-SDH gene in mutant white-fleshed loquat |
| title_short | The variation of NAD+-SDH gene in mutant white-fleshed loquat |
| title_sort | variation of nad sdh gene in mutant white fleshed loquat |
| topic | loquat flesh color NAD+-SDH SNP fructose |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612977 |
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