‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress
Global climate change is leading to more frequent extreme cold events, underscoring the need to study citrus cold tolerance to support breeding and enable potential northward expansion of citrus cultivation. In this study, the ‘Miyagawa’ wild type and its cold-tolerant mutant were selected for syste...
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MDPI AG
2025-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/7/1570 |
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| author | Shuangyou Wang Yingzi Zhang Ben Zhang Weiqi Luo Xiang Liu Suming Dai Dazhi Li Na Li |
| author_facet | Shuangyou Wang Yingzi Zhang Ben Zhang Weiqi Luo Xiang Liu Suming Dai Dazhi Li Na Li |
| author_sort | Shuangyou Wang |
| collection | DOAJ |
| description | Global climate change is leading to more frequent extreme cold events, underscoring the need to study citrus cold tolerance to support breeding and enable potential northward expansion of citrus cultivation. In this study, the ‘Miyagawa’ wild type and its cold-tolerant mutant were selected for systematic comparison across cold-resistant phenotypes, leaf tissue structure, physiological and biochemical characteristics, and <i data-eusoft-scrollable-element="1">Cor8</i> gene expression. The mutant exhibited 50% lower relative conductivity and malondialdehyde (MDA) content under −6 °C stress compared to the wild type, indicating reduced membrane damage. Antioxidant enzyme activities were significantly higher in the mutant: superoxide dismutase (SOD) activity increased by 10–30%, peroxidase (POD) by 28%, and catalase (CAT) by up to 2-fold. Proline content was 57% higher in the mutant at peak levels, supporting stronger osmotic regulation. Moreover, <i data-eusoft-scrollable-element="1">Cor8</i> gene expression in the mutant was up to 2.98 times higher than in the wild type during natural overwintering. These findings confirm that the ‘Miyagawa’ mutant possesses distinct physiological, anatomical, and molecular advantages for low-temperature adaptation and provides valuable germplasm for breeding cold-tolerant citrus varieties. |
| format | Article |
| id | doaj-art-291f94e6bccb43c587ba4b8cb7ce7c8a |
| institution | Matheson Library |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-291f94e6bccb43c587ba4b8cb7ce7c8a2025-07-25T13:09:49ZengMDPI AGAgronomy2073-43952025-06-01157157010.3390/agronomy15071570‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature StressShuangyou Wang0Yingzi Zhang1Ben Zhang2Weiqi Luo3Xiang Liu4Suming Dai5Dazhi Li6Na Li7College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCenter for Integrated Pest Management, North Carolina State University, Raleigh, NC 27606, USACollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaGlobal climate change is leading to more frequent extreme cold events, underscoring the need to study citrus cold tolerance to support breeding and enable potential northward expansion of citrus cultivation. In this study, the ‘Miyagawa’ wild type and its cold-tolerant mutant were selected for systematic comparison across cold-resistant phenotypes, leaf tissue structure, physiological and biochemical characteristics, and <i data-eusoft-scrollable-element="1">Cor8</i> gene expression. The mutant exhibited 50% lower relative conductivity and malondialdehyde (MDA) content under −6 °C stress compared to the wild type, indicating reduced membrane damage. Antioxidant enzyme activities were significantly higher in the mutant: superoxide dismutase (SOD) activity increased by 10–30%, peroxidase (POD) by 28%, and catalase (CAT) by up to 2-fold. Proline content was 57% higher in the mutant at peak levels, supporting stronger osmotic regulation. Moreover, <i data-eusoft-scrollable-element="1">Cor8</i> gene expression in the mutant was up to 2.98 times higher than in the wild type during natural overwintering. These findings confirm that the ‘Miyagawa’ mutant possesses distinct physiological, anatomical, and molecular advantages for low-temperature adaptation and provides valuable germplasm for breeding cold-tolerant citrus varieties.https://www.mdpi.com/2073-4395/15/7/1570citrusmutantlow temperature stressphysiology and biochemistry |
| spellingShingle | Shuangyou Wang Yingzi Zhang Ben Zhang Weiqi Luo Xiang Liu Suming Dai Dazhi Li Na Li ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress Agronomy citrus mutant low temperature stress physiology and biochemistry |
| title | ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress |
| title_full | ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress |
| title_fullStr | ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress |
| title_full_unstemmed | ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress |
| title_short | ‘Miyagawa’ New Bud Mutant Type: Enhances Resistance to Low-Temperature Stress |
| title_sort | miyagawa new bud mutant type enhances resistance to low temperature stress |
| topic | citrus mutant low temperature stress physiology and biochemistry |
| url | https://www.mdpi.com/2073-4395/15/7/1570 |
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