Effects of the Novel <i>Lacticaseibacillus paracasei</i> K-68 Inoculant on Nutrient Content, Fermentation, and Microbial Dynamics Changes in Dacheongok Corn Silage
This study investigated the role of <i>Lacticaseibacillus paracasei</i> K-68 (LABK) and cocktail LAB (LABC) as silage inoculants to enhance corn silage fermentation quality and microbial stability. Silage spoilage is primarily caused by undesirable microbes such as <i>Clostridium&l...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/6/304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study investigated the role of <i>Lacticaseibacillus paracasei</i> K-68 (LABK) and cocktail LAB (LABC) as silage inoculants to enhance corn silage fermentation quality and microbial stability. Silage spoilage is primarily caused by undesirable microbes such as <i>Clostridium</i>, <i>Klebsiella</i>, yeasts, and molds. The isolated LAB strain K-68 exhibited strong antibacterial and antifungal activity, particularly against spoilage organisms, and was identified as <i>L. paracasei</i>. Experimental silages inoculated with LABK or a LABC significantly improved fermentation profiles, with reduced pH and increased lactic acid levels. Microbial counts revealed that LAB-inoculated silages had higher LAB counts and significantly reduced yeast and mold populations. Furthermore, there were no significant differences in acetic acid, isobutyric acid, and propionic acid levels. High-throughput sequencing confirmed that LABK-treated silage was dominated by <i>Lacticaseibacillus paracasei</i>, whereas LABC-treated silage supported more diverse microbiota, including <i>Pediococcus pentosaceus</i>, <i>Lacrimispora xylanolytica</i>, and <i>Levilactobacillus brevis</i>. Both treatments suppressed spoilage-associated genera such as <i>Clostridium</i> and <i>Klebsiella.</i> Furthermore, correlation analysis showed that <i>Lacticaseibacillus</i> abundance was positively associated with lactic acid production and negatively correlated with pH and yeast levels. <i>L. paracasei</i> K-68 is a promising bio-inoculant for corn silage production since it promotes beneficial microbial dominance and suppresses spoilage organisms better than cocktail LAB. |
|---|---|
| ISSN: | 2311-5637 |