Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker
<i>Aeromonas salmonicida</i> is a common spoilage bacterium found in refrigerated fish. In this study, a virulent bacteriophage was isolated from wastewater using <i>A. salmonicida</i> AS08 as the host, and it was designated as TSW001. Based on morphological characterization...
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2025-06-01
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| author | Jun Yan Zhenghao Guo Jing Xie |
| author_facet | Jun Yan Zhenghao Guo Jing Xie |
| author_sort | Jun Yan |
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| description | <i>Aeromonas salmonicida</i> is a common spoilage bacterium found in refrigerated fish. In this study, a virulent bacteriophage was isolated from wastewater using <i>A. salmonicida</i> AS08 as the host, and it was designated as TSW001. Based on morphological characterization and whole-genome analysis, bacteriophage TSW001 was classified within the genus <i>Tedavirus</i>. Biological characterization revealed that TSW001 maintained a stable titer within a temperature range of 4~60 °C, a pH range of 4~9, and a salinity range of 50~1000 mM. The optimal multiplicity of infection (MOI) for TSW001 was 0.1, with a short latency period of approximately 10 min and a burst size of approximately 68 PFU/cell. When applied during the cold storage of large yellow croaker, the <i>A. salmonicida</i> count in the fish juice decreased by approximately 2.1~2.3 log<sub>10</sub> CFU/mL over the first two days, while the count in the fish fillets decreased by approximately 1.1~1.8 log<sub>10</sub> CFU/g. Furthermore, TSW001 demonstrated the ability to inhibit the formation of <i>A. salmonicida</i> biofilms. These results suggest that phage TSW001 is a promising biological antimicrobial agent for controlling <i>A. salmonicida</i> during the cold storage of seafood. |
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| spelling | doaj-art-27ab28f200f34c2080b33ec4ab07f9672025-06-25T13:50:50ZengMDPI AGFoods2304-81582025-06-011412208210.3390/foods14122082Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow CroakerJun Yan0Zhenghao Guo1Jing Xie2College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China<i>Aeromonas salmonicida</i> is a common spoilage bacterium found in refrigerated fish. In this study, a virulent bacteriophage was isolated from wastewater using <i>A. salmonicida</i> AS08 as the host, and it was designated as TSW001. Based on morphological characterization and whole-genome analysis, bacteriophage TSW001 was classified within the genus <i>Tedavirus</i>. Biological characterization revealed that TSW001 maintained a stable titer within a temperature range of 4~60 °C, a pH range of 4~9, and a salinity range of 50~1000 mM. The optimal multiplicity of infection (MOI) for TSW001 was 0.1, with a short latency period of approximately 10 min and a burst size of approximately 68 PFU/cell. When applied during the cold storage of large yellow croaker, the <i>A. salmonicida</i> count in the fish juice decreased by approximately 2.1~2.3 log<sub>10</sub> CFU/mL over the first two days, while the count in the fish fillets decreased by approximately 1.1~1.8 log<sub>10</sub> CFU/g. Furthermore, TSW001 demonstrated the ability to inhibit the formation of <i>A. salmonicida</i> biofilms. These results suggest that phage TSW001 is a promising biological antimicrobial agent for controlling <i>A. salmonicida</i> during the cold storage of seafood.https://www.mdpi.com/2304-8158/14/12/2082large yellow croaker<i>Aeromonas salmonicida</i>phagebiocontrolbiofilm |
| spellingShingle | Jun Yan Zhenghao Guo Jing Xie Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker Foods large yellow croaker <i>Aeromonas salmonicida</i> phage biocontrol biofilm |
| title | Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker |
| title_full | Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker |
| title_fullStr | Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker |
| title_full_unstemmed | Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker |
| title_short | Isolation and Characterization of <i>Aeromonas salmonicida</i> Phage TSW001 and Its Application on Large Yellow Croaker |
| title_sort | isolation and characterization of i aeromonas salmonicida i phage tsw001 and its application on large yellow croaker |
| topic | large yellow croaker <i>Aeromonas salmonicida</i> phage biocontrol biofilm |
| url | https://www.mdpi.com/2304-8158/14/12/2082 |
| work_keys_str_mv | AT junyan isolationandcharacterizationofiaeromonassalmonicidaiphagetsw001anditsapplicationonlargeyellowcroaker AT zhenghaoguo isolationandcharacterizationofiaeromonassalmonicidaiphagetsw001anditsapplicationonlargeyellowcroaker AT jingxie isolationandcharacterizationofiaeromonassalmonicidaiphagetsw001anditsapplicationonlargeyellowcroaker |