<i>Vibrio cholerae</i> O1 Avirulent Strains - Producers of Protective O1 Antigen: Obtaining and Peculiarities
Among 15 Vibrio cholerae El Tor O1 virulent strains of Inaba and Ogawa serovars grown in sterile river water, 6 strains were found to lose spontaneously the core region of CTXφ prophage including structural genes (ctxAB) of cholera toxin. It was determined that the obtained ctxA- mutants did not pro...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2010-08-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/562 |
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| Summary: | Among 15 Vibrio cholerae El Tor O1 virulent strains of Inaba and Ogawa serovars grown in sterile river water, 6 strains were found to lose spontaneously the core region of CTXφ prophage including structural genes (ctxAB) of cholera toxin. It was determined that the obtained ctxA- mutants did not produce cholera toxin and did not cause specific choleragenic reaction in the intra-intestinally inoculated suckling rabbits suggesting that they belonged to the IIId pathogenicity group. Analysis of the population structure of two selected non-toxigenic strains M569A and 5/65A revealed clones with high level of O1 antigen of Inaba and Ogawa serovars production. The obtained avirulent strains can be used as producers of above-noted protective antigens both in production of chemical cholera vaccine and in constructing of diagnostic immunoenzyme test-systems. |
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| ISSN: | 0370-1069 2658-719X |