Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album

Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album

The objects of the study were recombinant clones of Komagataella phaffii K51 carrying the heterologous proteinase K (PK-w) gene from Tritirachium album integrated into their genome as well as samples of recombinant proteinase K isolated from these clones. The aims of this work were i) to determine w...

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Bibliographic Details
Main Authors: A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2022-01-01
Series:Вавиловский журнал генетики и селекции
Subjects:
proteinase k
gene cloning
komagataella phaffii
gene expression
enzymatic activity
Online Access:https://vavilov.elpub.ru/jour/article/view/3207
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