HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation
[Objective] To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. [Methods] HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using...
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| Format: | Article |
| Language: | Chinese |
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-07-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.017&lang=en |
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| _version_ | 1839581967784345600 |
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| author | QUAN Zhanrou LIU Jie YANG Bingna ZHANG Yinming ZOU Hongyan |
| author_facet | QUAN Zhanrou LIU Jie YANG Bingna ZHANG Yinming ZOU Hongyan |
| author_sort | QUAN Zhanrou |
| collection | DOAJ |
| description | [Objective] To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. [Methods] HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using PCR-sequence-specific oligonucleotide probes (PCR-SSOP) and next-generation sequencing (NGS). Additionally, STR analysis was conducted on these samples using a 21-locus STR assay kit to detect STR loci. [Results] The HLA typing and STR locus outcomes of the patient's peripheral blood and the second saliva sample post-transplantation were in full concordance with the test results of the donor (father), whereas the HLA typing and STR locus results derived from the buccal swabs and the first saliva sample indicated chimerism between the donor and the recipient. [Conclusion] In the follow-up and monitoring after transplantation, apart from focusing on peripheral blood samples, it is recommended to regularly monitor HLA typing and STR loci chimerism in patients' buccal swabs and saliva samples to comprehensively evaluate the transplantation effect and recurrence risk. |
| format | Article |
| id | doaj-art-1fe88b62c35e45c38a545dfd53ba31f1 |
| institution | Matheson Library |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-07-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-1fe88b62c35e45c38a545dfd53ba31f12025-08-04T08:37:28ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-07-0138797597910.13303/j.cjbt.issn.1004-549x.2025.07.0171004-549X(2025)7-0975-06HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantationQUAN Zhanrou0LIU Jie1YANG Bingna2ZHANG Yinming3ZOU Hongyan4Institute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, ChinaInstitute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, ChinaInstitute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, ChinaInstitute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, ChinaInstitute of Transfusion Medicine, Shenzhen Blood Center, Shenzhen 518040, China[Objective] To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. [Methods] HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using PCR-sequence-specific oligonucleotide probes (PCR-SSOP) and next-generation sequencing (NGS). Additionally, STR analysis was conducted on these samples using a 21-locus STR assay kit to detect STR loci. [Results] The HLA typing and STR locus outcomes of the patient's peripheral blood and the second saliva sample post-transplantation were in full concordance with the test results of the donor (father), whereas the HLA typing and STR locus results derived from the buccal swabs and the first saliva sample indicated chimerism between the donor and the recipient. [Conclusion] In the follow-up and monitoring after transplantation, apart from focusing on peripheral blood samples, it is recommended to regularly monitor HLA typing and STR loci chimerism in patients' buccal swabs and saliva samples to comprehensively evaluate the transplantation effect and recurrence risk.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.017&lang=enhla typingstr locihematopoietic stem cell transplantationacute lymphoblastic leukemiachimerism |
| spellingShingle | QUAN Zhanrou LIU Jie YANG Bingna ZHANG Yinming ZOU Hongyan HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation Zhongguo shuxue zazhi hla typing str loci hematopoietic stem cell transplantation acute lymphoblastic leukemia chimerism |
| title | HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation |
| title_full | HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation |
| title_fullStr | HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation |
| title_full_unstemmed | HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation |
| title_short | HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation |
| title_sort | hla typing and str loci chimerism analysis of a case of acute lymphoblastic leukemia after hla haploidentical hematopoietic stem cell transplantation |
| topic | hla typing str loci hematopoietic stem cell transplantation acute lymphoblastic leukemia chimerism |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.017&lang=en |
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