HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation

HLA typing and STR loci chimerism analysis of a case of acute lymphoblastic leukemia after HLA haploidentical hematopoietic stem cell transplantation

[Objective] To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. [Methods] HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using...

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Bibliographic Details
Main Authors: QUAN Zhanrou, LIU Jie, YANG Bingna, ZHANG Yinming, ZOU Hongyan
Format: Article
Language:Chinese
Published: Institute of Blood Transfusion of Chinese Academy of Medical Sciences 2025-07-01
Series:Zhongguo shuxue zazhi
Subjects:
hla typing
str loci
hematopoietic stem cell transplantation
acute lymphoblastic leukemia
chimerism
Online Access:https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.017&lang=en
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Summary:[Objective] To analyze the HLA typing and STR loci chimerism in a patient with recurrent acute lymphoblastic leukemia after HLA-haploidentical hematopoietic stem cell transplantation. [Methods] HLA typing was performed on peripheral blood, buccal swabs and saliva samples after transplantation using PCR-sequence-specific oligonucleotide probes (PCR-SSOP) and next-generation sequencing (NGS). Additionally, STR analysis was conducted on these samples using a 21-locus STR assay kit to detect STR loci. [Results] The HLA typing and STR locus outcomes of the patient's peripheral blood and the second saliva sample post-transplantation were in full concordance with the test results of the donor (father), whereas the HLA typing and STR locus results derived from the buccal swabs and the first saliva sample indicated chimerism between the donor and the recipient. [Conclusion] In the follow-up and monitoring after transplantation, apart from focusing on peripheral blood samples, it is recommended to regularly monitor HLA typing and STR loci chimerism in patients' buccal swabs and saliva samples to comprehensively evaluate the transplantation effect and recurrence risk.
ISSN:1004-549X

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