Molecular diagnostic techniques in respiratory infections
The diagnosis of community-acquired pneumonia and hospital-acquired pneumonia is based on clinical, radiological, and microbiological findings. Conventional methods are based on the culture of respiratory samples, including sputum, endotracheal aspirate, bronchoaspirate, and bronchoalveolar lavage,...
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| Format: | Article |
| Language: | English |
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Publicaciones Permanyer
2025-04-01
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| Series: | Barcelona Respiratory Network Reviews |
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| Online Access: | https://www.brnreviews.com/frame_eng.php?id=276 |
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| author | Jordi Vila-Estapé Arturo Martínez-Trejo Giulia Gatti Natalia Roson-Calero Laia Fernandez-Barat Antoni Torres Andrea Vergara-Gómez |
| author_facet | Jordi Vila-Estapé Arturo Martínez-Trejo Giulia Gatti Natalia Roson-Calero Laia Fernandez-Barat Antoni Torres Andrea Vergara-Gómez |
| author_sort | Jordi Vila-Estapé |
| collection | DOAJ |
| description | The diagnosis of community-acquired pneumonia and hospital-acquired pneumonia is based on clinical, radiological, and microbiological findings. Conventional methods are based on the culture of respiratory samples, including sputum, endotracheal aspirate, bronchoaspirate, and bronchoalveolar lavage, followed by strain identification and antibiotic susceptibility testing. The gold standard for microbiological diagnosis of pneumonia remains the culture-based methods. These take > 24 h to identify the bacteria and 48 h to provide antibacterial susceptibility. Culture is insensitive, only detecting a pathogen in 23-40% of patients with clinically diagnosed pneumonia and an even smaller proportion after the administration of antibiotics. The utilization of multiplex panels for the simultaneous detection and identification of respiratory pathogens, including the detection of resistant determinants, can streamline testing procedures and enhance both the sensitivity and speed of diagnosis compared to traditional. In this review, molecular testing is currently available, and the potential future applications of next-generation sequencing are developed.
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| format | Article |
| id | doaj-art-1f8f0941b5af44dd9dd5fef94e28a305 |
| institution | Matheson Library |
| issn | 2462-3172 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Publicaciones Permanyer |
| record_format | Article |
| series | Barcelona Respiratory Network Reviews |
| spelling | doaj-art-1f8f0941b5af44dd9dd5fef94e28a3052025-07-10T21:57:14ZengPublicaciones PermanyerBarcelona Respiratory Network Reviews2462-31722025-04-0111210.23866/BRNRev:2025-M0131Molecular diagnostic techniques in respiratory infectionsJordi Vila-Estapé0Arturo Martínez-Trejo1Giulia Gatti2Natalia Roson-Calero3Laia Fernandez-Barat4Antoni Torres5Andrea Vergara-Gómez6Department of Clinical Microbiology, Hospital Clínic, Barcelona, Spain; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; School of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto Salud Carlos III, Madrid. SpainBarcelona Institute for Global Health (ISGlobal), Barcelona, Spain; School of Medicine and Health Sciences, University of Barcelona, Barcelona, SpainDepartment of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum-University of Bologna, Bologna, ItalyBarcelona Institute for Global Health (ISGlobal), Barcelona, Spain; School of Medicine and Health Sciences, University of Barcelona, Barcelona, SpainSchool of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; Cellex Research Laboratories, CIBERES (Center for Net Biomedical Research Respiratory Diseases), Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain Respiratory Intensive Care Unit, Department of Pneumology, Hospital Clínic, Barcelona, Spain.Pneumology Service, Hospital Clínic, Barcelna; Faculty of Medicine, University of Barcelona, Spain; Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS), Barcelona; CIBER de enfermedades respiratorias (Ciberes), SpainDepartment of Clinical Microbiology, Hospital Clínic, Barcelona, Spain; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; School of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain; CIBER de Enfermedades Infecciosas (CIBERINFEC), Instituto Salud Carlos III, Madrid. SpainThe diagnosis of community-acquired pneumonia and hospital-acquired pneumonia is based on clinical, radiological, and microbiological findings. Conventional methods are based on the culture of respiratory samples, including sputum, endotracheal aspirate, bronchoaspirate, and bronchoalveolar lavage, followed by strain identification and antibiotic susceptibility testing. The gold standard for microbiological diagnosis of pneumonia remains the culture-based methods. These take > 24 h to identify the bacteria and 48 h to provide antibacterial susceptibility. Culture is insensitive, only detecting a pathogen in 23-40% of patients with clinically diagnosed pneumonia and an even smaller proportion after the administration of antibiotics. The utilization of multiplex panels for the simultaneous detection and identification of respiratory pathogens, including the detection of resistant determinants, can streamline testing procedures and enhance both the sensitivity and speed of diagnosis compared to traditional. In this review, molecular testing is currently available, and the potential future applications of next-generation sequencing are developed. https://www.brnreviews.com/frame_eng.php?id=276Diagnostics. Respiratory tract infections. Molecular diagnosis. Conventional diagnosis. |
| spellingShingle | Jordi Vila-Estapé Arturo Martínez-Trejo Giulia Gatti Natalia Roson-Calero Laia Fernandez-Barat Antoni Torres Andrea Vergara-Gómez Molecular diagnostic techniques in respiratory infections Barcelona Respiratory Network Reviews Diagnostics. Respiratory tract infections. Molecular diagnosis. Conventional diagnosis. |
| title | Molecular diagnostic techniques in respiratory infections |
| title_full | Molecular diagnostic techniques in respiratory infections |
| title_fullStr | Molecular diagnostic techniques in respiratory infections |
| title_full_unstemmed | Molecular diagnostic techniques in respiratory infections |
| title_short | Molecular diagnostic techniques in respiratory infections |
| title_sort | molecular diagnostic techniques in respiratory infections |
| topic | Diagnostics. Respiratory tract infections. Molecular diagnosis. Conventional diagnosis. |
| url | https://www.brnreviews.com/frame_eng.php?id=276 |
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