Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice
ObjectiveTo explore the role and related mechanism of neutrophil membrane-coated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Neu-NP) in cardiac repair after acute myocardial ischemia-reperfusion (MI/R) injury in mice. MethodsThe male C57 mouse model of acute MI/R injury was established and r...
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Shanghai Chinese Clinical Medicine Press Co., Ltd.
2025-06-01
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| Series: | Zhongguo Linchuang Yixue |
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| Online Access: | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250194 |
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| author | Jing CHEN Yanan SONG Zheyong HUANG Junbo GE |
| author_facet | Jing CHEN Yanan SONG Zheyong HUANG Junbo GE |
| author_sort | Jing CHEN |
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| description | ObjectiveTo explore the role and related mechanism of neutrophil membrane-coated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Neu-NP) in cardiac repair after acute myocardial ischemia-reperfusion (MI/R) injury in mice. MethodsThe male C57 mouse model of acute MI/R injury was established and randomly divided into three groups: PBS control group (injection of 200 μL PBS), NP treatment group (injection of 0.5 mg/mL NP 200 μL), and Neu-NP treatment group (injection of 0.5 mg/mL Neu-NP 200 μL). Neutrophil membranes were extracted and fused with PLGA nanoparticles to construct biomimetic Neu-NP. The in vivo homing ability of Neu-NP was assessed using ex vivo imaging technology in the MI/R injury model, and the expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the myocardium were measured using enzyme linked immunosorbent assay one day and three days after administration. Echocardiography was used to determine cardiac function indicators of MI/R injured mice 28 days post-administration. Immunofluorescence staining was used to observe angiogenesis repair and inflammatory cell infiltration in mouse heart tissue. ResultsNeu-NP, engineered by integrating neutrophil membranes with nanoparticles, inherited surface receptors (TNF-αR and IL-6R) and functioned as decoys for inflammatory targeting. Compared with the PBS control group and NP treatment group, the secretion levels of TNF-α and IL-6 in the damaged myocardium of the Neu-NP treatment group were significantly decreased one and three days after administration (P＜0.05); 28 days after administration, the cardiac ejection fraction in the Neu-NP treatment group was significantly higher than that in the other two groups (P＜0.05). Immunofluorescence staining indicated a significant increase in the proportion of angiogenesis in the myocardial infarction area and a significant reduction in inflammation cell infiltration (P＜0.05). ConclusionsNeu-NP plays an important role in cardiac tissue repair after MI/R injury by alleviating inflammatory factors in the damaged area and promoting angiogenesis. |
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| institution | Matheson Library |
| issn | 1008-6358 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Shanghai Chinese Clinical Medicine Press Co., Ltd. |
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| spelling | doaj-art-1f0c60ea640f4a5f814b40a23b49f1772025-07-10T05:43:05ZengShanghai Chinese Clinical Medicine Press Co., Ltd.Zhongguo Linchuang Yixue1008-63582025-06-0132338439110.12025/j.issn.1008-6358.2025.2025019420250194Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in miceJing CHEN0Yanan SONG1Zheyong HUANG2Junbo GE3Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, State Key Laboratory of Cardiology, National Clinical Research Center for Interventional Medicine, Shanghai Clinical Research Center for Interventional Medicine, Shanghai 200032, ChinaDepartment of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, State Key Laboratory of Cardiology, National Clinical Research Center for Interventional Medicine, Shanghai Clinical Research Center for Interventional Medicine, Shanghai 200032, ChinaDepartment of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, State Key Laboratory of Cardiology, National Clinical Research Center for Interventional Medicine, Shanghai Clinical Research Center for Interventional Medicine, Shanghai 200032, ChinaDepartment of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, State Key Laboratory of Cardiology, National Clinical Research Center for Interventional Medicine, Shanghai Clinical Research Center for Interventional Medicine, Shanghai 200032, ChinaObjectiveTo explore the role and related mechanism of neutrophil membrane-coated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (Neu-NP) in cardiac repair after acute myocardial ischemia-reperfusion (MI/R) injury in mice. MethodsThe male C57 mouse model of acute MI/R injury was established and randomly divided into three groups: PBS control group (injection of 200 μL PBS), NP treatment group (injection of 0.5 mg/mL NP 200 μL), and Neu-NP treatment group (injection of 0.5 mg/mL Neu-NP 200 μL). Neutrophil membranes were extracted and fused with PLGA nanoparticles to construct biomimetic Neu-NP. The in vivo homing ability of Neu-NP was assessed using ex vivo imaging technology in the MI/R injury model, and the expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the myocardium were measured using enzyme linked immunosorbent assay one day and three days after administration. Echocardiography was used to determine cardiac function indicators of MI/R injured mice 28 days post-administration. Immunofluorescence staining was used to observe angiogenesis repair and inflammatory cell infiltration in mouse heart tissue. ResultsNeu-NP, engineered by integrating neutrophil membranes with nanoparticles, inherited surface receptors (TNF-αR and IL-6R) and functioned as decoys for inflammatory targeting. Compared with the PBS control group and NP treatment group, the secretion levels of TNF-α and IL-6 in the damaged myocardium of the Neu-NP treatment group were significantly decreased one and three days after administration (P＜0.05); 28 days after administration, the cardiac ejection fraction in the Neu-NP treatment group was significantly higher than that in the other two groups (P＜0.05). Immunofluorescence staining indicated a significant increase in the proportion of angiogenesis in the myocardial infarction area and a significant reduction in inflammation cell infiltration (P＜0.05). ConclusionsNeu-NP plays an important role in cardiac tissue repair after MI/R injury by alleviating inflammatory factors in the damaged area and promoting angiogenesis.https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250194neutrophil membrane-coated nanoparticlesmyocardial ischemia-reperfusioninflammation neutralizationtissue repair |
| spellingShingle | Jing CHEN Yanan SONG Zheyong HUANG Junbo GE Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice Zhongguo Linchuang Yixue neutrophil membrane-coated nanoparticles myocardial ischemia-reperfusion inflammation neutralization tissue repair |
| title | Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice |
| title_full | Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice |
| title_fullStr | Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice |
| title_full_unstemmed | Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice |
| title_short | Neutrophil membrane-coated PLGA nanoparticles promoting the repair of myocardial ischemia-reperfusion injury in mice |
| title_sort | neutrophil membrane coated plga nanoparticles promoting the repair of myocardial ischemia reperfusion injury in mice |
| topic | neutrophil membrane-coated nanoparticles myocardial ischemia-reperfusion inflammation neutralization tissue repair |
| url | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20250194 |
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