Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i>
Since rice blast is a disease that causes significant damage to rice all over the world, the intensive development of disease-resistant genotypes is of great urgency. The use of molecular biology technologies, new biotechnological approaches and molecular genetic methods allows creating disease-resis...
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
2024-11-01
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| Series: | Зерновое хозяйство России |
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| Online Access: | https://www.zhros.online/jour/article/view/2815 |
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| author | O. V. Shumskaya N. N. Vozhzhova O. S. Zhogaleva Yu. A. Khakhulina P. I. Kostylev |
| author_facet | O. V. Shumskaya N. N. Vozhzhova O. S. Zhogaleva Yu. A. Khakhulina P. I. Kostylev |
| author_sort | O. V. Shumskaya |
| collection | DOAJ |
| description | Since rice blast is a disease that causes significant damage to rice all over the world, the intensive development of disease-resistant genotypes is of great urgency. The use of molecular biology technologies, new biotechnological approaches and molecular genetic methods allows creating disease-resistant varieties while reducing the time of their development, which plays an important role in growing competitive products. The current paper has highlighted the results of estimating rice samples developed at the FSBSI “ARC “Donskoy” for blast resistance. The objects of the study were new samples developed in the laboratory of rice breeding and seed production. In 2022–2023, there were screened one hundred eighty-seven rice samples. They were identified on a presence of the blast resistance gene Pi-2. One hundred fifteen samples were estimated for the blast resistance gene Pi-b. Genomic DNA was isolated from young rice leaves. After estimating the quantity and quality of the isolated DNA there was conducted a polymerase chain reaction for the target genes Pi-2 and Pi-b. In order to identify the alleles of the desired genes there were used specific intragenic molecular SSR markers of dominant (for Pi-2) and codominant (for Pi-b) types. Electrophoresis of the obtained amplicons was performed on 2 % agarose gels during an hour. The results of the analysis were recorded in ultraviolet light and processed in ImageLab 5.1 software. As a result of the conducted study, there were identified new rice breeding samples that carry blast resistance genes Pi-2 and Pi-b. These samples are recommended for use in the further breeding process for blast resistance. |
| format | Article |
| id | doaj-art-1eed1e5514c64c73b2e1eb4f26e4b24b |
| institution | Matheson Library |
| issn | 2079-8725 2079-8733 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" |
| record_format | Article |
| series | Зерновое хозяйство России |
| spelling | doaj-art-1eed1e5514c64c73b2e1eb4f26e4b24b2025-08-03T19:54:01ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332024-11-01165717710.31367/2079-8725-2024-94-5-71-77983Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i>O. V. Shumskaya0N. N. Vozhzhova1O. S. Zhogaleva2Yu. A. Khakhulina3P. I. Kostylev4FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”FSBSI Agricultural Research Center “Donskoy”Since rice blast is a disease that causes significant damage to rice all over the world, the intensive development of disease-resistant genotypes is of great urgency. The use of molecular biology technologies, new biotechnological approaches and molecular genetic methods allows creating disease-resistant varieties while reducing the time of their development, which plays an important role in growing competitive products. The current paper has highlighted the results of estimating rice samples developed at the FSBSI “ARC “Donskoy” for blast resistance. The objects of the study were new samples developed in the laboratory of rice breeding and seed production. In 2022–2023, there were screened one hundred eighty-seven rice samples. They were identified on a presence of the blast resistance gene Pi-2. One hundred fifteen samples were estimated for the blast resistance gene Pi-b. Genomic DNA was isolated from young rice leaves. After estimating the quantity and quality of the isolated DNA there was conducted a polymerase chain reaction for the target genes Pi-2 and Pi-b. In order to identify the alleles of the desired genes there were used specific intragenic molecular SSR markers of dominant (for Pi-2) and codominant (for Pi-b) types. Electrophoresis of the obtained amplicons was performed on 2 % agarose gels during an hour. The results of the analysis were recorded in ultraviolet light and processed in ImageLab 5.1 software. As a result of the conducted study, there were identified new rice breeding samples that carry blast resistance genes Pi-2 and Pi-b. These samples are recommended for use in the further breeding process for blast resistance.https://www.zhros.online/jour/article/view/2815ricerice blastmarkergeneresistance<i>pi-2</i><i>pi-b</i> |
| spellingShingle | O. V. Shumskaya N. N. Vozhzhova O. S. Zhogaleva Yu. A. Khakhulina P. I. Kostylev Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> Зерновое хозяйство России rice rice blast marker gene resistance <i>pi-2</i> <i>pi-b</i> |
| title | Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> |
| title_full | Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> |
| title_fullStr | Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> |
| title_full_unstemmed | Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> |
| title_short | Identification of rice samples with rice blast resistance genes <i>Pi-2</i> and <i>Pi-b</i> |
| title_sort | identification of rice samples with rice blast resistance genes i pi 2 i and i pi b i |
| topic | rice rice blast marker gene resistance <i>pi-2</i> <i>pi-b</i> |
| url | https://www.zhros.online/jour/article/view/2815 |
| work_keys_str_mv | AT ovshumskaya identificationofricesampleswithriceblastresistancegenesipi2iandipibi AT nnvozhzhova identificationofricesampleswithriceblastresistancegenesipi2iandipibi AT oszhogaleva identificationofricesampleswithriceblastresistancegenesipi2iandipibi AT yuakhakhulina identificationofricesampleswithriceblastresistancegenesipi2iandipibi AT pikostylev identificationofricesampleswithriceblastresistancegenesipi2iandipibi |