Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007
The study showed that blaCTX-M genes were present in the genomes of 71% of сephаlosporin resistant Enterobacteriaceae nosocomial isolates (n=833) collected in Russian hospitals within 2003-2007, including 91% of E.coli, 90% of Klebsiella spp., 38% of Enterobacter spp., 31 % of Citrobacter spp. (n=9)...
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LLC "Publishing House OKI"
2020-05-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/338 |
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| author | N. K. Fursova S. D. Pryamchuk I. V. Abaev YU. N. Kovalev N. A. Shishkova E. I. Pecherskikh O. V. Korobova E. I. Astashkin D. M. Pachkunov E. A. Svetoch S. V. Sidorenko |
| author_facet | N. K. Fursova S. D. Pryamchuk I. V. Abaev YU. N. Kovalev N. A. Shishkova E. I. Pecherskikh O. V. Korobova E. I. Astashkin D. M. Pachkunov E. A. Svetoch S. V. Sidorenko |
| author_sort | N. K. Fursova |
| collection | DOAJ |
| description | The study showed that blaCTX-M genes were present in the genomes of 71% of сephаlosporin resistant Enterobacteriaceae nosocomial isolates (n=833) collected in Russian hospitals within 2003-2007, including 91% of E.coli, 90% of Klebsiella spp., 38% of Enterobacter spp., 31 % of Citrobacter spp. (n=9), and 36% of the other Enterobacteriaceae species. The genes belonging to the following subtypes (clusters) were identified: blaCΎX_M_1 (529 blaCTX_M_15 genes; 25 blaCTX_M_3 genes; 1 blaCTX_M_22 gene, 1 blaCTX-M-23 gene and 1 blaCTX-M-34 gene); blaCTX-M-2 (1 blaCTX-M-2 gene and 4 blaCTX-M-5 genes), and blaCTX-M-9 (2 blaCTX-M_9 genes, and 28 blaCTX_M_14 genes). It was shown that blaCTX-M genes were located on high-molecular weight (60-160 bp) conjugative plasmids belonging mainly to the incompatibility groups IncF, IncL/M and IncA/C (blaCTX-M-15 gene); IncL/M (blaCTX-M-3 gene); and IncF, IncL/M and IncIl-ly (CTX-M-14 gene). The gene environments of blaCTX-M genes were shown specific for the subtype of the genes. A mobile genetic element lSEcp1 (in some cases deleted or inserted by IS26, IS1, IS10, resTn2, or resTn3 sequences, in direct or reverse position) were detected upstream of blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15 genes. A special characteristic was the sequence between ISEcpl and blaCTX-M gene: 48 bp for blaCTX-M-15 (except 1 E.coli isolate having such a sequence deleted by 3 bp); 127 bp for blaCTX-M-3; 42 bp for blaCTX-M-14. Downstream of blaCTX-M and blaCTX-M-15 genes in the major bacterial isolates orf477 mucA and Aorf477-AmucA sequences were detected respectively. Two isolates had additional Aorf3 insertion inside of Aorf477-AmucA sequence. Insertion sequence IS903 (intact or deleted) was detected downstream of blaCTX-M-14 gene. Unlike the others, blaCTX-M-2 and blaCTX-M-9 genes were located inside of IS CR1 mobile element, downstream of class 1 integron and orf513 sequence. |
| format | Article |
| id | doaj-art-1e8c1ee48b0f406f81f6b2c8e63bfb70 |
| institution | Matheson Library |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | LLC "Publishing House OKI" |
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| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-1e8c1ee48b0f406f81f6b2c8e63bfb702025-08-03T13:04:46ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-015511-12310337Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007N. K. Fursova0S. D. Pryamchuk1I. V. Abaev2YU. N. Kovalev3N. A. Shishkova4E. I. Pecherskikh5O. V. Korobova6E. I. Astashkin7D. M. Pachkunov8E. A. Svetoch9S. V. Sidorenko10State Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskState Research Centre for Applied Microbiology and Biotechnology, ObolenskResearch Institute for Children' Infections, St.PetersburgThe study showed that blaCTX-M genes were present in the genomes of 71% of сephаlosporin resistant Enterobacteriaceae nosocomial isolates (n=833) collected in Russian hospitals within 2003-2007, including 91% of E.coli, 90% of Klebsiella spp., 38% of Enterobacter spp., 31 % of Citrobacter spp. (n=9), and 36% of the other Enterobacteriaceae species. The genes belonging to the following subtypes (clusters) were identified: blaCΎX_M_1 (529 blaCTX_M_15 genes; 25 blaCTX_M_3 genes; 1 blaCTX_M_22 gene, 1 blaCTX-M-23 gene and 1 blaCTX-M-34 gene); blaCTX-M-2 (1 blaCTX-M-2 gene and 4 blaCTX-M-5 genes), and blaCTX-M-9 (2 blaCTX-M_9 genes, and 28 blaCTX_M_14 genes). It was shown that blaCTX-M genes were located on high-molecular weight (60-160 bp) conjugative plasmids belonging mainly to the incompatibility groups IncF, IncL/M and IncA/C (blaCTX-M-15 gene); IncL/M (blaCTX-M-3 gene); and IncF, IncL/M and IncIl-ly (CTX-M-14 gene). The gene environments of blaCTX-M genes were shown specific for the subtype of the genes. A mobile genetic element lSEcp1 (in some cases deleted or inserted by IS26, IS1, IS10, resTn2, or resTn3 sequences, in direct or reverse position) were detected upstream of blaCTX-M-3, blaCTX-M-14, and blaCTX-M-15 genes. A special characteristic was the sequence between ISEcpl and blaCTX-M gene: 48 bp for blaCTX-M-15 (except 1 E.coli isolate having such a sequence deleted by 3 bp); 127 bp for blaCTX-M-3; 42 bp for blaCTX-M-14. Downstream of blaCTX-M and blaCTX-M-15 genes in the major bacterial isolates orf477 mucA and Aorf477-AmucA sequences were detected respectively. Two isolates had additional Aorf3 insertion inside of Aorf477-AmucA sequence. Insertion sequence IS903 (intact or deleted) was detected downstream of blaCTX-M-14 gene. Unlike the others, blaCTX-M-2 and blaCTX-M-9 genes were located inside of IS CR1 mobile element, downstream of class 1 integron and orf513 sequence.https://www.antibiotics-chemotherapy.ru/jour/article/view/338bla<sub>ct</sub>x<sub>-</sub>misecplis26is903orf513iscrlbla<sub>ctx-m</sub>isecplis26is903orf513iscrl |
| spellingShingle | N. K. Fursova S. D. Pryamchuk I. V. Abaev YU. N. Kovalev N. A. Shishkova E. I. Pecherskikh O. V. Korobova E. I. Astashkin D. M. Pachkunov E. A. Svetoch S. V. Sidorenko Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 Антибиотики и Химиотерапия bla<sub>ct</sub>x<sub>-</sub>m isecpl is26 is903 orf513 iscrl bla<sub>ctx-m</sub> isecpl is26 is903 orf513 iscrl |
| title | Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 |
| title_full | Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 |
| title_fullStr | Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 |
| title_full_unstemmed | Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 |
| title_short | Genetic Environments of bla<sub>CTX-M</sub> Genes Located on Conjugative Plasmids of Enterobacteriaceae Nosocomial Isolates Collected in Russia within 2003-2007 |
| title_sort | genetic environments of bla sub ctx m sub genes located on conjugative plasmids of enterobacteriaceae nosocomial isolates collected in russia within 2003 2007 |
| topic | bla<sub>ct</sub>x<sub>-</sub>m isecpl is26 is903 orf513 iscrl bla<sub>ctx-m</sub> isecpl is26 is903 orf513 iscrl |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/338 |
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