Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay
Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Padjadjaran
2025-06-01
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| Series: | Althea Medical Journal |
| Subjects: | |
| Online Access: | https://journal.fk.unpad.ac.id/index.php/amj/article/view/3954 |
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| Summary: | Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research.
Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay.
Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness.
Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications. |
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| ISSN: | 2337-4330 |