Effects of silver nitrate on adventitious-buds formation in mulberry leaves cultured in vitro
The effects of AgNO<sub>3</sub> treatment on adventitious-buds formation in mulberry leaves cultured in vitro were studied. The results showed that compared with the control, treatment with addition of AgNO<sub>3</sub> to the culture media at the concentration of 1.0-2.0 mg·L...
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Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Zhejiang University Press
2000-01-01
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Series: | 浙江大学学报. 农业与生命科学版 |
Subjects: | |
Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.01.0097 |
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Summary: | The effects of AgNO<sub>3</sub> treatment on adventitious-buds formation in mulberry leaves cultured in vitro were studied. The results showed that compared with the control, treatment with addition of AgNO<sub>3</sub> to the culture media at the concentration of 1.0-2.0 mg·L<sup>-1</sup> presented a stable differentiation rate of 81.3%-87.5%, substantial increase of the clustered buds rate and 5-7 days earlier initiation of adventitious-buds differentiation. It was also found that the duration of culturing leaves on the media containing AgNO<sub>3</sub> should be no longer than 10 days, otherwise the frequency of differentiation would be reduced, and the cultures grew poorly, due to the toxic effect of Ag<sup>+</sup>. |
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ISSN: | 1008-9209 2097-5155 |