Rapid gas chromatogram determination of methane, organic acid in in vitro ruminal fermentation products
A rapid determination method of ruminal fermentation products by gas chromatogram has been developed. By using the HP-INNOWAX, FID and nitrogen as the carrier, the optimal condition for methane measurement was: nitrogen pressure at 130 kPa, total and column flow rates at 30.2 and 1.7 mL·min<sup&g...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.02.0217 |
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| Summary: | A rapid determination method of ruminal fermentation products by gas chromatogram has been developed. By using the HP-INNOWAX, FID and nitrogen as the carrier, the optimal condition for methane measurement was: nitrogen pressure at 130 kPa, total and column flow rates at 30.2 and 1.7 mL·min<sup>-1</sup>, and the column, injection and detection temperatures at 80, 100 and 120 ℃, respectively. The optimal condition for acetate, propionate and butyrate measurement was: nitrogen pressure at 90 kPa, total and column flow rates at 37.2 and 0.67 mL·min<sup>-1</sup>, and column, injection and detection temperatures at 180, 200 and 220 ℃, respectively. |
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| ISSN: | 1008-9209 2097-5155 |