Spectrophtometric determination of Hydrochlorothiazaide by oxidative coupling with O-Phenylendiamine
This study includes the development of a new sensitive spectrophotometric method for the determination of a hydrochlorothaizide in aqueous solution and pharmaceultical preparations. The method is based on the oxidative coupling reaction of hydrochlorothaizide with O-phenylendiamine reagent in a aci...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Tikrit University
2019-04-01
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| Series: | Tikrit Journal of Pure Science |
| Subjects: | |
| Online Access: | https://tjpsj.org/index.php/tjps/article/view/351 |
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| Summary: | This study includes the development of a new sensitive spectrophotometric method for the determination of a hydrochlorothaizide in aqueous solution and pharmaceultical preparations. The method is based on the oxidative coupling reaction of hydrochlorothaizide with O-phenylendiamine reagent in a acidic medium pH 1.5 in the presence of potassium ferricyanide to produce an intense orange color, water soluble and stable product , which exhibits maximum absorption at 450 nm. Beer’s law is obeyed over the concentration arange 6 to 48 µg.ml-1 of hydrochlorothaizide, with a molar absorptivity of 3602.17 L.mo1-1 .cm -1 , Sandell’s sensitivity index of 0.03 µg.cm-2 , relative error range not more than 0.215% , and D.L 0.0835µg.ml-1 . The method has been successfully applied for the determination of hydrochlorothaizide in tablets.
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| ISSN: | 1813-1662 2415-1726 |