Design of New Primer Sets for the Development of a Loop-Mediated Isothermal Amplification for Rapid Detection of <i>Neisseria meningitidis</i>
<i>Neisseria meningitidis</i> is a pathogenic bacterial agent that causes meningococcal meningitis in humans. Developing a rapid and low-cost <i>N. meningitidis</i> detection method is crucial, especially for developing countries. This study focuses on the development of an e...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/6/467 |
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| Summary: | <i>Neisseria meningitidis</i> is a pathogenic bacterial agent that causes meningococcal meningitis in humans. Developing a rapid and low-cost <i>N. meningitidis</i> detection method is crucial, especially for developing countries. This study focuses on the development of an efficient loop-mediated isothermal amplification (LAMP) method for accurate <i>N. meningitidis</i> identification. A new LAMP primer set was designed, and a LAMP reaction was optimized. The colorimetric detection method was also applied, and the assay characteristics were evaluated using clinical samples. The results demonstrated a specific LAMP reaction for <i>N. meningitidis</i> detection of genotypes A, B, and C, with a limit of detection of 10<sup>2</sup> cfu/mL, 100% specificity and sensitivity, and a rapid detection time of only 40 min by colorimetric visual inspection. No cross-reactivity with reference strains of <i>Streptococcus pneumoniae</i>, <i>Staphylococcus aureus</i>, <i>Neisseria lactamica</i>, <i>Mycobacterium tuberculosis</i>, and <i>Haemophilus influenzae</i> type b was observed in the LAMP reaction with the new primer set. This result suggests that the LAMP reaction could be a promising tool for developing a rapid <i>N. meningitidis</i> detection method suitable for use in Vietnam and other developing countries. |
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| ISSN: | 1467-3037 1467-3045 |