Isolation of live human δ cells for genetic and functional analysis
Investigations of human pancreatic islets have been empowered by strategies to isolate and study live islet cell subsets, like β cells and α cells. To advance experimentation with human islet δ cells, which remain relatively understudied, we generated combinatorial cell sorting approaches to separat...
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| Κύριοι συγγραφείς: | , , , , |
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| Μορφή: | Άρθρο |
| Γλώσσα: | Αγγλικά |
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Elsevier
2025-08-01
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| Σειρά: | Molecular Metabolism |
| Θέματα: | |
| Διαθέσιμο Online: | http://www.sciencedirect.com/science/article/pii/S221287782500095X |
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| _version_ | 1839635662941192192 |
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| author | Yan Hang Mario Alex Miranda Ziqiao Yan Weichen Zhao Seung K. Kim |
| author_facet | Yan Hang Mario Alex Miranda Ziqiao Yan Weichen Zhao Seung K. Kim |
| author_sort | Yan Hang |
| collection | DOAJ |
| description | Investigations of human pancreatic islets have been empowered by strategies to isolate and study live islet cell subsets, like β cells and α cells. To advance experimentation with human islet δ cells, which remain relatively understudied, we generated combinatorial cell sorting approaches to separate human δ cells from β cells, yielding highly-enriched human δ cells. We used molecular analysis, immunohistology, and electroporation-based targeting to demonstrate the quality of δ cell purification. We also demonstrated the feasibility of prospectively studying human δ cell function in pseudoislet organoids. Innovations detailed here should promote discovery of genetic, signaling and physiological mechanisms governing δ cell function and roles in human islets. |
| format | Article |
| id | doaj-art-119ad0ecc7b242baa65d31022c5dc058 |
| institution | Matheson Library |
| issn | 2212-8778 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj-art-119ad0ecc7b242baa65d31022c5dc0582025-07-09T04:32:21ZengElsevierMolecular Metabolism2212-87782025-08-0198102188Isolation of live human δ cells for genetic and functional analysisYan Hang0Mario Alex Miranda1Ziqiao Yan2Weichen Zhao3Seung K. Kim4Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA, 94305, USA; Corresponding authors. Department of Developmental Biology, Stanford University School of Medicine, 279 Campus Drive, B300, Stanford, CA, 94305, USA.Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USADepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USADepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USADepartment of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA, 94305, USA; Department of Medicine (Endocrinology Division), Stanford University School of Medicine, Stanford, CA, 94305, USA; Department of Pediatrics (Endocrinology Division), Stanford University School of Medicine, Stanford, CA, 94305, USA; Corresponding authors. Department of Developmental Biology, Stanford University School of Medicine, 279 Campus Drive, B300, Stanford, CA, 94305, USA.Investigations of human pancreatic islets have been empowered by strategies to isolate and study live islet cell subsets, like β cells and α cells. To advance experimentation with human islet δ cells, which remain relatively understudied, we generated combinatorial cell sorting approaches to separate human δ cells from β cells, yielding highly-enriched human δ cells. We used molecular analysis, immunohistology, and electroporation-based targeting to demonstrate the quality of δ cell purification. We also demonstrated the feasibility of prospectively studying human δ cell function in pseudoislet organoids. Innovations detailed here should promote discovery of genetic, signaling and physiological mechanisms governing δ cell function and roles in human islets.http://www.sciencedirect.com/science/article/pii/S221287782500095XHuman islet: δ cellsβ cellsα cellsPseudoisletPancreasDiabetes |
| spellingShingle | Yan Hang Mario Alex Miranda Ziqiao Yan Weichen Zhao Seung K. Kim Isolation of live human δ cells for genetic and functional analysis Molecular Metabolism Human islet: δ cells β cells α cells Pseudoislet Pancreas Diabetes |
| title | Isolation of live human δ cells for genetic and functional analysis |
| title_full | Isolation of live human δ cells for genetic and functional analysis |
| title_fullStr | Isolation of live human δ cells for genetic and functional analysis |
| title_full_unstemmed | Isolation of live human δ cells for genetic and functional analysis |
| title_short | Isolation of live human δ cells for genetic and functional analysis |
| title_sort | isolation of live human δ cells for genetic and functional analysis |
| topic | Human islet: δ cells β cells α cells Pseudoislet Pancreas Diabetes |
| url | http://www.sciencedirect.com/science/article/pii/S221287782500095X |
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