TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken
The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important inte...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001496 |
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| author | Huan-xian CUI Na LUO Li-ping GUO Lu LIU Si-yuan XING Gui-ping ZHAO Jie WEN |
| author_facet | Huan-xian CUI Na LUO Li-ping GUO Lu LIU Si-yuan XING Gui-ping ZHAO Jie WEN |
| author_sort | Huan-xian CUI |
| collection | DOAJ |
| description | The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2 (TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated (P<0.01) in muscle tissue of individuals with high intramuscular fat content. Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat, C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism (such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction (such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated (P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content. Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway. |
| format | Article |
| id | doaj-art-10f1fa88d67f4cee9c6e0c3f32b8edb3 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-10f1fa88d67f4cee9c6e0c3f32b8edb32025-07-02T02:05:36ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-03-01223853863TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chickenHuan-xian CUI0Na LUO1Li-ping GUO2Lu LIU3Si-yuan XING4Gui-ping ZHAO5Jie WEN6State Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaState Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaCorrespondence ZHAO Gui-ping; State Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaCorrespondence WEN Jie; State Key Laboratory of Animal Nutrition/Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.ChinaThe interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2 (TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated (P<0.01) in muscle tissue of individuals with high intramuscular fat content. Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat, C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism (such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction (such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated (P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content. Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway.http://www.sciencedirect.com/science/article/pii/S2095311922001496TIMP2ECMintramuscular adipocytesmuscle satellite cellschicken |
| spellingShingle | Huan-xian CUI Na LUO Li-ping GUO Lu LIU Si-yuan XING Gui-ping ZHAO Jie WEN TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken Journal of Integrative Agriculture TIMP2 ECM intramuscular adipocytes muscle satellite cells chicken |
| title | TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| title_full | TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| title_fullStr | TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| title_full_unstemmed | TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| title_short | TIMP2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| title_sort | timp2 promotes intramuscular fat deposition by regulating the extracellular matrix in chicken |
| topic | TIMP2 ECM intramuscular adipocytes muscle satellite cells chicken |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001496 |
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