Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates
<b>Background/Objective</b>: Platelet concentrates (PCs) are used in transfusion medicine to treat bleeding disorders. <i>Staphylococcus aureus</i>, a predominant PC contaminant, has been implicated in several adverse transfusion reactions. The aim of this study was to invest...
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2025-06-01
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| author | Carina Paredes Que Chi Truong-Bolduc Yin Wang David C. Hooper Sandra Ramirez-Arcos |
| author_facet | Carina Paredes Que Chi Truong-Bolduc Yin Wang David C. Hooper Sandra Ramirez-Arcos |
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| description | <b>Background/Objective</b>: Platelet concentrates (PCs) are used in transfusion medicine to treat bleeding disorders. <i>Staphylococcus aureus</i>, a predominant PC contaminant, has been implicated in several adverse transfusion reactions. The aim of this study was to investigate the impact of PC storage on <i>S. aureus</i> resistance to quinolones, which are commonly used to treat <i>S. aureus</i> infections. <b>Methods/Results</b>: Four transfusion-relevant <i>S. aureus</i> strains (TRSs) were subjected to comparative transcriptome analyses when grown in PCs vs. trypticase soy broth (TSB). Results of these analyses revealed differentially expressed genes involved in antibiotic resistance. Of interest, the <i>norB</i> gene (encodes for the NorB efflux pump, which is implicated in quinolone resistance and is negatively regulated by MgrA) was upregulated (1.2–4.7-fold increase) in all PC-grown TRS compared to TSB cultures. Minimal Bactericidal Concentration (MBC) of ciprofloxacin and norfloxacin in PC-grown TRS compared to TSB showed increased resistance to both quinolones in PC cultures. Complementary studies with non-transfusion-relevant strains <i>S. aureus</i> RN6390 and its <i>norB</i> and <i>mgrA</i> deletion mutants were conducted. MBC of ciprofloxacin and norfloxacin and RT-qPCR assays of these strains showed that not only <i>norB</i>, but also <i>norA</i> and <i>norC</i> may be involved in enhanced quinolone resistance in PC-grown <i>S. aureus</i>. The role of <i>norB</i> in <i>S. aureus</i> virulence was also tested using the silkworm <i>Bombyx mori</i> animal model; lethal dose 50 (LD<sub>50</sub>) assays revealed slightly higher virulence in larvae infected with the wild-type strain compared to the <i>norB</i> mutant. <b>Conclusions</b>: The PC storage environment enhances quinolone resistance in <i>S. aureus</i> and induces differential expression of efflux pump <i>nor</i> genes. Furthermore, our preliminary data of the involvement of NorB in virulence of <i>S. aureus</i> using a silkworm model merit further investigation with other systems such as a mammal animal model. Our results provide mechanistic insights to aid clinicians in the selection of antimicrobial treatment of patients receiving transfusions of <i>S. aureus</i>-contaminated PCs. |
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| spelling | doaj-art-10ce6c03f8d94d7dbee6a7dfb813f5d72025-07-25T13:11:14ZengMDPI AGAntibiotics2079-63822025-06-0114763510.3390/antibiotics14070635Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet ConcentratesCarina Paredes0Que Chi Truong-Bolduc1Yin Wang2David C. Hooper3Sandra Ramirez-Arcos4Department of Biochemistry, Microbiology, and Immunology (BMI), Faculty of Medicine, University of Ottawa, Ottawa, ON K1G 4J5, CanadaDivision of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USADivision of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USADivision of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USADepartment of Biochemistry, Microbiology, and Immunology (BMI), Faculty of Medicine, University of Ottawa, Ottawa, ON K1G 4J5, Canada<b>Background/Objective</b>: Platelet concentrates (PCs) are used in transfusion medicine to treat bleeding disorders. <i>Staphylococcus aureus</i>, a predominant PC contaminant, has been implicated in several adverse transfusion reactions. The aim of this study was to investigate the impact of PC storage on <i>S. aureus</i> resistance to quinolones, which are commonly used to treat <i>S. aureus</i> infections. <b>Methods/Results</b>: Four transfusion-relevant <i>S. aureus</i> strains (TRSs) were subjected to comparative transcriptome analyses when grown in PCs vs. trypticase soy broth (TSB). Results of these analyses revealed differentially expressed genes involved in antibiotic resistance. Of interest, the <i>norB</i> gene (encodes for the NorB efflux pump, which is implicated in quinolone resistance and is negatively regulated by MgrA) was upregulated (1.2–4.7-fold increase) in all PC-grown TRS compared to TSB cultures. Minimal Bactericidal Concentration (MBC) of ciprofloxacin and norfloxacin in PC-grown TRS compared to TSB showed increased resistance to both quinolones in PC cultures. Complementary studies with non-transfusion-relevant strains <i>S. aureus</i> RN6390 and its <i>norB</i> and <i>mgrA</i> deletion mutants were conducted. MBC of ciprofloxacin and norfloxacin and RT-qPCR assays of these strains showed that not only <i>norB</i>, but also <i>norA</i> and <i>norC</i> may be involved in enhanced quinolone resistance in PC-grown <i>S. aureus</i>. The role of <i>norB</i> in <i>S. aureus</i> virulence was also tested using the silkworm <i>Bombyx mori</i> animal model; lethal dose 50 (LD<sub>50</sub>) assays revealed slightly higher virulence in larvae infected with the wild-type strain compared to the <i>norB</i> mutant. <b>Conclusions</b>: The PC storage environment enhances quinolone resistance in <i>S. aureus</i> and induces differential expression of efflux pump <i>nor</i> genes. Furthermore, our preliminary data of the involvement of NorB in virulence of <i>S. aureus</i> using a silkworm model merit further investigation with other systems such as a mammal animal model. Our results provide mechanistic insights to aid clinicians in the selection of antimicrobial treatment of patients receiving transfusions of <i>S. aureus</i>-contaminated PCs.https://www.mdpi.com/2079-6382/14/7/635<i>Staphylococcus aureus</i>platelet concentratesquinolone resistance by <i>Staphylococcus aureus</i><i>Staphylococcus aureus</i> virulencesilkworm animal model |
| spellingShingle | Carina Paredes Que Chi Truong-Bolduc Yin Wang David C. Hooper Sandra Ramirez-Arcos Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates Antibiotics <i>Staphylococcus aureus</i> platelet concentrates quinolone resistance by <i>Staphylococcus aureus</i> <i>Staphylococcus aureus</i> virulence silkworm animal model |
| title | Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates |
| title_full | Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates |
| title_fullStr | Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates |
| title_full_unstemmed | Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates |
| title_short | Enhanced Quinolone Resistance and Differential Expression of Efflux Pump <i>nor</i> Genes in <i>Staphylococcus aureus</i> Grown in Platelet Concentrates |
| title_sort | enhanced quinolone resistance and differential expression of efflux pump i nor i genes in i staphylococcus aureus i grown in platelet concentrates |
| topic | <i>Staphylococcus aureus</i> platelet concentrates quinolone resistance by <i>Staphylococcus aureus</i> <i>Staphylococcus aureus</i> virulence silkworm animal model |
| url | https://www.mdpi.com/2079-6382/14/7/635 |
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