Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro
Porcine reproductive and respiratory syndrome virus (PRRSV) infection has inflicted devastating impacts on the global swine industry, while current vaccines provide limited protection against this disease. Mogroside V (MV), a triterpenoid compound derived from Siraitia grosvenorii, exhibits diverse...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611600/full |
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| author | Longhua Liang Longhua Liang Xiaohui Huang Xiaohui Huang Guoxi Qin Guoxi Qin Xiaoyu Ma Rijing He Rijing He Hongpeng Dai Hongpeng Dai Zhen Zhang Zhen Zhang Xiaogan Yang Xiaogan Yang Xingwei Liang Xingwei Liang |
| author_facet | Longhua Liang Longhua Liang Xiaohui Huang Xiaohui Huang Guoxi Qin Guoxi Qin Xiaoyu Ma Rijing He Rijing He Hongpeng Dai Hongpeng Dai Zhen Zhang Zhen Zhang Xiaogan Yang Xiaogan Yang Xingwei Liang Xingwei Liang |
| author_sort | Longhua Liang |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) infection has inflicted devastating impacts on the global swine industry, while current vaccines provide limited protection against this disease. Mogroside V (MV), a triterpenoid compound derived from Siraitia grosvenorii, exhibits diverse biological activities including antioxidant, anti-inflammatory, and anti-cancer properties, with the capacity to scavenge free radicals and mitigate oxidative stress. In this study, MV was administered to PRRSV-infected cells via three distinct treatment modalities. Our findings demonstrate that MV effectively blocks or suppresses infections caused by diverse PRRSV subtypes in porcine alveolar macrophages (PAMs) and Marc-145 cells. MV exhibited significant dose-dependent antiviral efficacy, with viral titers and mRNA expression inhibited by over 90% at a concentration of 400 μM. Comparative analysis further revealed substantial variations in antiviral efficacy among the different treatment protocols. Notably, PRRSV employs immune evasion mechanisms to suppress host innate immunity. MV not only directly inhibited PRRSV replication but also significantly upregulated the gene expression of immunomodulatory cytokines (IL-1, IL-2, IL-8, IL-18; P < 0.05), suggesting a dual mechanism of antiviral action. These findings underscore the antiviral bioactivity of MV and highlight its potential as a novel therapeutic candidate for PRRSV intervention. |
| format | Article |
| id | doaj-art-10399a6f757241bd9d836f8045cc76c2 |
| institution | Matheson Library |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-10399a6f757241bd9d836f8045cc76c22025-07-02T05:51:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16116001611600Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitroLonghua Liang0Longhua Liang1Xiaohui Huang2Xiaohui Huang3Guoxi Qin4Guoxi Qin5Xiaoyu Ma6Rijing He7Rijing He8Hongpeng Dai9Hongpeng Dai10Zhen Zhang11Zhen Zhang12Xiaogan Yang13Xiaogan Yang14Xingwei Liang15Xingwei Liang16College of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Agricultural Reclamation Yongxin Livestock Group Jinguang Livestock Co., Ltd, Nanning, ChinaGuangxi Agricultural Reclamation Yongxin Livestock Group Jinguang Livestock Co., Ltd, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning, ChinaGuangxi Key Laboratory of Animal Breeding & Disease Control and Prevention, Guangxi University, Nanning, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) infection has inflicted devastating impacts on the global swine industry, while current vaccines provide limited protection against this disease. Mogroside V (MV), a triterpenoid compound derived from Siraitia grosvenorii, exhibits diverse biological activities including antioxidant, anti-inflammatory, and anti-cancer properties, with the capacity to scavenge free radicals and mitigate oxidative stress. In this study, MV was administered to PRRSV-infected cells via three distinct treatment modalities. Our findings demonstrate that MV effectively blocks or suppresses infections caused by diverse PRRSV subtypes in porcine alveolar macrophages (PAMs) and Marc-145 cells. MV exhibited significant dose-dependent antiviral efficacy, with viral titers and mRNA expression inhibited by over 90% at a concentration of 400 μM. Comparative analysis further revealed substantial variations in antiviral efficacy among the different treatment protocols. Notably, PRRSV employs immune evasion mechanisms to suppress host innate immunity. MV not only directly inhibited PRRSV replication but also significantly upregulated the gene expression of immunomodulatory cytokines (IL-1, IL-2, IL-8, IL-18; P < 0.05), suggesting a dual mechanism of antiviral action. These findings underscore the antiviral bioactivity of MV and highlight its potential as a novel therapeutic candidate for PRRSV intervention.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611600/fullPRRSVmogroside Vantiviral actionantioxidantcytokines |
| spellingShingle | Longhua Liang Longhua Liang Xiaohui Huang Xiaohui Huang Guoxi Qin Guoxi Qin Xiaoyu Ma Rijing He Rijing He Hongpeng Dai Hongpeng Dai Zhen Zhang Zhen Zhang Xiaogan Yang Xiaogan Yang Xingwei Liang Xingwei Liang Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro Frontiers in Microbiology PRRSV mogroside V antiviral action antioxidant cytokines |
| title | Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro |
| title_full | Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro |
| title_fullStr | Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro |
| title_full_unstemmed | Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro |
| title_short | Antiviral effects of mogroside V against porcine reproductive and respiratory syndrome virus in vitro |
| title_sort | antiviral effects of mogroside v against porcine reproductive and respiratory syndrome virus in vitro |
| topic | PRRSV mogroside V antiviral action antioxidant cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611600/full |
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