Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i>
<i>Brassica</i> crops, cultivated as vegetables, oilseeds, and forages, are vital economic resources in agricultural production. However, black rot caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) poses a significant threat to...
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2025-06-01
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| Series: | Horticulturae |
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| author | Siping Deng Congcong Kong Hongxue Ma Jialei Ji Yong Wang Yangyong Zhang Mu Zhuang Limei Yang Zhiyuan Fang Vasiliy Taranov Anna M. Artemyeva Honghao Lv |
| author_facet | Siping Deng Congcong Kong Hongxue Ma Jialei Ji Yong Wang Yangyong Zhang Mu Zhuang Limei Yang Zhiyuan Fang Vasiliy Taranov Anna M. Artemyeva Honghao Lv |
| author_sort | Siping Deng |
| collection | DOAJ |
| description | <i>Brassica</i> crops, cultivated as vegetables, oilseeds, and forages, are vital economic resources in agricultural production. However, black rot caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) poses a significant threat to the production of these crops. This study aimed to enhance the resistance resource pool for <i>Brassica</i> crops by evaluating 29 inbred lines and 52 commercial cultivars of <i>B. rapa</i> through an inoculation test. Among these, 11 inbred lines, such as ‘E5’ and ‘LW’, and 8 commercial cultivars, such as ‘QX’ and ‘SY’, demonstrated high resistance. We constructed a genetic segregating population (P<sub>1</sub>, P<sub>2</sub>, F<sub>1</sub>, F<sub>2</sub>) using the highly resistant line ‘E5’ and the highly susceptible line ‘E4’ as parents. Utilizing a major gene plus polygenic mixed inheritance model for genetic analysis, our findings indicate that the resistance to black rot in ‘E5’ is governed by a pair of additive-dominant polygenes, and the main gene heritability is 93.43%. Furthermore, transmission electron microscopy examination revealed numerous autophagic structures in the xylem parenchyma cells of the highly resistant line ‘E5’, while the highly susceptible line exhibited cell necrosis, indicating that the resistant material might protect mesophyll cells and adjacent structures through programmed cell death. This research contributes novel genetic materials for breeding disease-resistant varieties, enhances our understanding of <i>Xcc</i> invasion mechanisms and host defense traits, and establishes a theoretical framework for the effective prevention and control of these diseases. |
| format | Article |
| id | doaj-art-0e7ff0fd50524b738c96e4bb79a5f44f |
| institution | Matheson Library |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-0e7ff0fd50524b738c96e4bb79a5f44f2025-06-25T13:55:18ZengMDPI AGHorticulturae2311-75242025-06-0111662610.3390/horticulturae11060626Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i>Siping Deng0Congcong Kong1Hongxue Ma2Jialei Ji3Yong Wang4Yangyong Zhang5Mu Zhuang6Limei Yang7Zhiyuan Fang8Vasiliy Taranov9Anna M. Artemyeva10Honghao Lv11State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaAll-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Science, Moscow 195251, RussiaFederal Research Center N.I. Vavilow All-Russian Institute of Plant Genetic Resources, St. Petersburg 190000, RussiaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China<i>Brassica</i> crops, cultivated as vegetables, oilseeds, and forages, are vital economic resources in agricultural production. However, black rot caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) poses a significant threat to the production of these crops. This study aimed to enhance the resistance resource pool for <i>Brassica</i> crops by evaluating 29 inbred lines and 52 commercial cultivars of <i>B. rapa</i> through an inoculation test. Among these, 11 inbred lines, such as ‘E5’ and ‘LW’, and 8 commercial cultivars, such as ‘QX’ and ‘SY’, demonstrated high resistance. We constructed a genetic segregating population (P<sub>1</sub>, P<sub>2</sub>, F<sub>1</sub>, F<sub>2</sub>) using the highly resistant line ‘E5’ and the highly susceptible line ‘E4’ as parents. Utilizing a major gene plus polygenic mixed inheritance model for genetic analysis, our findings indicate that the resistance to black rot in ‘E5’ is governed by a pair of additive-dominant polygenes, and the main gene heritability is 93.43%. Furthermore, transmission electron microscopy examination revealed numerous autophagic structures in the xylem parenchyma cells of the highly resistant line ‘E5’, while the highly susceptible line exhibited cell necrosis, indicating that the resistant material might protect mesophyll cells and adjacent structures through programmed cell death. This research contributes novel genetic materials for breeding disease-resistant varieties, enhances our understanding of <i>Xcc</i> invasion mechanisms and host defense traits, and establishes a theoretical framework for the effective prevention and control of these diseases.https://www.mdpi.com/2311-7524/11/6/626black rotgermplasm screeninginheritance analysiscellular mechanism |
| spellingShingle | Siping Deng Congcong Kong Hongxue Ma Jialei Ji Yong Wang Yangyong Zhang Mu Zhuang Limei Yang Zhiyuan Fang Vasiliy Taranov Anna M. Artemyeva Honghao Lv Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> Horticulturae black rot germplasm screening inheritance analysis cellular mechanism |
| title | Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> |
| title_full | Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> |
| title_fullStr | Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> |
| title_full_unstemmed | Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> |
| title_short | Genetic and Cellular Basis of Resistance to Black Rot Caused by <i>Xanthomonas campestris</i> pv. <i>campestris</i> in <i>Brassica rapa</i> |
| title_sort | genetic and cellular basis of resistance to black rot caused by i xanthomonas campestris i pv i campestris i in i brassica rapa i |
| topic | black rot germplasm screening inheritance analysis cellular mechanism |
| url | https://www.mdpi.com/2311-7524/11/6/626 |
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