Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus
The novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of vira...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1582 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839615502192738304 |
|---|---|
| author | Yimin Wang Yong Wang Zhuangli Bi Jinbin Wang Gang Wang Xin Ru Chunchun Meng Jie Zhu Guangqing Liu Chuanfeng Li |
| author_facet | Yimin Wang Yong Wang Zhuangli Bi Jinbin Wang Gang Wang Xin Ru Chunchun Meng Jie Zhu Guangqing Liu Chuanfeng Li |
| author_sort | Yimin Wang |
| collection | DOAJ |
| description | The novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of viral infections are critical for preventing the spread of epidemics. In this study, a TaqMan probe-based duplex one-step RT-qPCR was established for the simultaneous detection and qualitative and quantitative identification of the two viruses. It demonstrated greater sensitivity than conventional PCR, detecting as low as 2.42 copies/μL of NGPV genome and 70.1 copies/μL of NDRV genome. Additionally, it exhibited remarkable specificity, responding exclusively to the nucleic acids of target pathogens. It also demonstrated excellent reproducibility and availability, particularly in clinical settings, with a coinfection detection rate of 13.3%, contributing to the development of NGPV- and NDRV-testing technologies. |
| format | Article |
| id | doaj-art-0e6c0c5c23ae4c4dbb1d43a18d38b8db |
| institution | Matheson Library |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-0e6c0c5c23ae4c4dbb1d43a18d38b8db2025-07-25T13:31:09ZengMDPI AGMicroorganisms2076-26072025-07-01137158210.3390/microorganisms13071582Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck ReovirusYimin Wang0Yong Wang1Zhuangli Bi2Jinbin Wang3Gang Wang4Xin Ru5Chunchun Meng6Jie Zhu7Guangqing Liu8Chuanfeng Li9Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaThe novel goose parvovirus (NGPV) and the novel duck reovirus (NDRV) are pathogens that can substantially affect the growth and development of ducklings, causing considerable economic losses to duck farms. Therefore, a timely, rapid, accurate, and high-throughput diagnosis and identification of viral infections are critical for preventing the spread of epidemics. In this study, a TaqMan probe-based duplex one-step RT-qPCR was established for the simultaneous detection and qualitative and quantitative identification of the two viruses. It demonstrated greater sensitivity than conventional PCR, detecting as low as 2.42 copies/μL of NGPV genome and 70.1 copies/μL of NDRV genome. Additionally, it exhibited remarkable specificity, responding exclusively to the nucleic acids of target pathogens. It also demonstrated excellent reproducibility and availability, particularly in clinical settings, with a coinfection detection rate of 13.3%, contributing to the development of NGPV- and NDRV-testing technologies.https://www.mdpi.com/2076-2607/13/7/1582novel goose parvovirusnovel duck reovirussimultaneous detectionone-step qPCR |
| spellingShingle | Yimin Wang Yong Wang Zhuangli Bi Jinbin Wang Gang Wang Xin Ru Chunchun Meng Jie Zhu Guangqing Liu Chuanfeng Li Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus Microorganisms novel goose parvovirus novel duck reovirus simultaneous detection one-step qPCR |
| title | Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus |
| title_full | Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus |
| title_fullStr | Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus |
| title_full_unstemmed | Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus |
| title_short | Development of a TaqMan One-Step Quantitative PCR Assay for the Simultaneous Detection of Novel Goose Parvovirus and Novel Duck Reovirus |
| title_sort | development of a taqman one step quantitative pcr assay for the simultaneous detection of novel goose parvovirus and novel duck reovirus |
| topic | novel goose parvovirus novel duck reovirus simultaneous detection one-step qPCR |
| url | https://www.mdpi.com/2076-2607/13/7/1582 |
| work_keys_str_mv | AT yiminwang developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT yongwang developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT zhuanglibi developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT jinbinwang developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT gangwang developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT xinru developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT chunchunmeng developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT jiezhu developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT guangqingliu developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus AT chuanfengli developmentofataqmanonestepquantitativepcrassayforthesimultaneousdetectionofnovelgooseparvovirusandnovelduckreovirus |