Comparison of esterase isozymes in two color varieties of Tenebrio molitor L
In order to provide some references for studying the genetic variation and selecting varieties of Tenebrio molitor, the esterase isozyme zymograms and activity at different developmental stages of the yellow- and black-color varieties of T. molitor were compared using vertical polyacrylamide gel ele...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.06.004 |
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| Summary: | In order to provide some references for studying the genetic variation and selecting varieties of Tenebrio molitor, the esterase isozyme zymograms and activity at different developmental stages of the yellow- and black-color varieties of T. molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry. The results showed that 15 enzyme bands with different mobility were found at all, which were distributed in five zones. The larvae and adults of the black-color variety showed one more distinctive band compared with those of the yellow larvae and adults, respectively. Moreover, band numbers, migration rate of enzyme bands and strength of enzymatic activity at different developmental stages of both color varieties varied greatly. Compared with other developmental stage, band numbers of esterase isozyme were the least (only four bands) and enzymatic activity was the weakest at egg stage, and two of four bands were found only at this stage. The enzymatic activities of carboxylesterase (CarE), acetylcholine esterase (AchE), acid phosphatase (ACP) and alkaline phosphatase (AKP) in black-color variety were always higher than those in yellow-color variety at all stages from egg to adult. While in the same variety, The enzymatic activity of CarE, AchE, ACP and AKP varied in the order of egg stage < adult stage < pupal stage < larval stage. The results above build up some underlying data for further studying the insecticide resistance and variety selection of the mealworm beetles. |
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| ISSN: | 1008-9209 2097-5155 |