Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation
<i>Mycogone perniciosa</i> is the causative agent of wet bubble disease, which induces significant losses in the production of <i>Agaricus bisporus</i>, indicating the high importance of the development of novel inhibitory agents. The isolation, identification, and molecular...
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2025-07-01
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| Series: | Journal of Fungi |
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| author | Jasmina Glamočlija Marija Ivanov Marina Soković Ana Ćirić Slavica Ninković Danijela Mišić Ivanka Milenković Dejan Stojković |
| author_facet | Jasmina Glamočlija Marija Ivanov Marina Soković Ana Ćirić Slavica Ninković Danijela Mišić Ivanka Milenković Dejan Stojković |
| author_sort | Jasmina Glamočlija |
| collection | DOAJ |
| description | <i>Mycogone perniciosa</i> is the causative agent of wet bubble disease, which induces significant losses in the production of <i>Agaricus bisporus</i>, indicating the high importance of the development of novel inhibitory agents. The isolation, identification, and molecular characterization of five isolates of <i>M. perniciosa</i> from diseased fruit bodies of <i>A. bisporus</i> was done. Moreover, the study evaluated the in vitro and in situ potential of <i>Origanum vulgare</i> essential oil (EO) to limit <i>M. perniciosa</i> growth and provided chemical characterization of its volatile components. The obtained strains differed phenotypically and according to their molecular characteristics. <i>O. vulgare</i> EO has shown more promising antifungal activity than the commercial fungicide Prochloraz-Mn in the microatmospheric method. In the treatment of experimentally induced wet bubble disease on <i>A. bisporus</i> in the growing chambers with 2% of <i>O. vulgare</i> EO and simultaneous application of spore suspension of mycopathogen, <i>O. vulgare</i> EO totally inhibited the growth of <i>M. perniciosa</i>. Carvacrol, p-cymene, γ-terpinene, and thymol were dominant constituents of <i>O. vulgare</i> EO examined in this study. <i>O. vulgare</i> EO has shown promising potential to limit growth of <i>M. perniciosa</i> and should be further explored as a novel biofungicide. |
| format | Article |
| id | doaj-art-0d76f50f5aa34dc39b4408e66f92fc8d |
| institution | Matheson Library |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-0d76f50f5aa34dc39b4408e66f92fc8d2025-07-25T13:27:22ZengMDPI AGJournal of Fungi2309-608X2025-07-0111751510.3390/jof11070515Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> CultivationJasmina Glamočlija0Marija Ivanov1Marina Soković2Ana Ćirić3Slavica Ninković4Danijela Mišić5Ivanka Milenković6Dejan Stojković7Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, SerbiaEkofungi, Zrenjaninski Put, Padinska Skela, 11213 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia<i>Mycogone perniciosa</i> is the causative agent of wet bubble disease, which induces significant losses in the production of <i>Agaricus bisporus</i>, indicating the high importance of the development of novel inhibitory agents. The isolation, identification, and molecular characterization of five isolates of <i>M. perniciosa</i> from diseased fruit bodies of <i>A. bisporus</i> was done. Moreover, the study evaluated the in vitro and in situ potential of <i>Origanum vulgare</i> essential oil (EO) to limit <i>M. perniciosa</i> growth and provided chemical characterization of its volatile components. The obtained strains differed phenotypically and according to their molecular characteristics. <i>O. vulgare</i> EO has shown more promising antifungal activity than the commercial fungicide Prochloraz-Mn in the microatmospheric method. In the treatment of experimentally induced wet bubble disease on <i>A. bisporus</i> in the growing chambers with 2% of <i>O. vulgare</i> EO and simultaneous application of spore suspension of mycopathogen, <i>O. vulgare</i> EO totally inhibited the growth of <i>M. perniciosa</i>. Carvacrol, p-cymene, γ-terpinene, and thymol were dominant constituents of <i>O. vulgare</i> EO examined in this study. <i>O. vulgare</i> EO has shown promising potential to limit growth of <i>M. perniciosa</i> and should be further explored as a novel biofungicide.https://www.mdpi.com/2309-608X/11/7/515<i>Mycogone perniciosa</i>RAPD<i>Agaricus bisporus</i><i>Origanum vulgare</i>in vitro and in situ fungicidal properties |
| spellingShingle | Jasmina Glamočlija Marija Ivanov Marina Soković Ana Ćirić Slavica Ninković Danijela Mišić Ivanka Milenković Dejan Stojković Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation Journal of Fungi <i>Mycogone perniciosa</i> RAPD <i>Agaricus bisporus</i> <i>Origanum vulgare</i> in vitro and in situ fungicidal properties |
| title | Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation |
| title_full | Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation |
| title_fullStr | Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation |
| title_full_unstemmed | Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation |
| title_short | Inhibitory Effects of <i>Origanum vulgare</i> Essential Oil on <i>Mycogone perniciosa</i> Growth in <i>Agaricus bisporus</i> Cultivation |
| title_sort | inhibitory effects of i origanum vulgare i essential oil on i mycogone perniciosa i growth in i agaricus bisporus i cultivation |
| topic | <i>Mycogone perniciosa</i> RAPD <i>Agaricus bisporus</i> <i>Origanum vulgare</i> in vitro and in situ fungicidal properties |
| url | https://www.mdpi.com/2309-608X/11/7/515 |
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