Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation
Since 1960s when transplantation was initiated in the world, pre formed HLA antibodies in the recipient’s serum against the donor have been associated with immediate allograft loss, inferior early and late graft outcomes. The immune system’s recognition of foreign HLA antigens can trigger an immune...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2025-04-01
|
| Series: | Indian Journal of Transplantation |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ijot.ijot_128_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839639647738658816 |
|---|---|
| author | Shruti Tapiawala Suchita Jogale Dolly Danielle Anil Handoo |
| author_facet | Shruti Tapiawala Suchita Jogale Dolly Danielle Anil Handoo |
| author_sort | Shruti Tapiawala |
| collection | DOAJ |
| description | Since 1960s when transplantation was initiated in the world, pre formed HLA antibodies in the recipient’s serum against the donor have been associated with immediate allograft loss, inferior early and late graft outcomes. The immune system’s recognition of foreign HLA antigens can trigger an immune response leading to graft rejection1. The necessity to identify those at risk of allograft loss and improving short term and long-term outcomes led to the development of crossmatch procedures. This test plays a crucial role in preventing transplant rejection by detecting pre-existing antibodies in the recipient that target donor HLA molecules. This article provides an overview of cell based crossmatches such as Complement Dependent cytotoxicity crossmatch (CDCXM) and Flow cytometry crossmatch (FCXM). The techniques, methodologies, advantages, limitations and application in day-to-day practice are discussed. The article will be helpful to both clinicians and laboratory personnel dealing with organ transplantation. |
| format | Article |
| id | doaj-art-0d4d4dd76d0a4474ba370ebb65e65fc8 |
| institution | Matheson Library |
| issn | 2212-0017 2212-0025 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Transplantation |
| spelling | doaj-art-0d4d4dd76d0a4474ba370ebb65e65fc82025-07-04T07:39:49ZengWolters Kluwer Medknow PublicationsIndian Journal of Transplantation2212-00172212-00252025-04-0119212513410.4103/ijot.ijot_128_24Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ TransplantationShruti TapiawalaSuchita JogaleDolly DanielleAnil HandooSince 1960s when transplantation was initiated in the world, pre formed HLA antibodies in the recipient’s serum against the donor have been associated with immediate allograft loss, inferior early and late graft outcomes. The immune system’s recognition of foreign HLA antigens can trigger an immune response leading to graft rejection1. The necessity to identify those at risk of allograft loss and improving short term and long-term outcomes led to the development of crossmatch procedures. This test plays a crucial role in preventing transplant rejection by detecting pre-existing antibodies in the recipient that target donor HLA molecules. This article provides an overview of cell based crossmatches such as Complement Dependent cytotoxicity crossmatch (CDCXM) and Flow cytometry crossmatch (FCXM). The techniques, methodologies, advantages, limitations and application in day-to-day practice are discussed. The article will be helpful to both clinicians and laboratory personnel dealing with organ transplantation.https://journals.lww.com/10.4103/ijot.ijot_128_24antihuman globulincomplement-dependent cytotoxicityflow cytometry crossmatchhuman leucocyte antigen |
| spellingShingle | Shruti Tapiawala Suchita Jogale Dolly Danielle Anil Handoo Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation Indian Journal of Transplantation antihuman globulin complement-dependent cytotoxicity flow cytometry crossmatch human leucocyte antigen |
| title | Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation |
| title_full | Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation |
| title_fullStr | Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation |
| title_full_unstemmed | Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation |
| title_short | Unfolding the Mysteries of Cell-based Human Leukocyte Antigen Crossmatching in Solid Organ Transplantation |
| title_sort | unfolding the mysteries of cell based human leukocyte antigen crossmatching in solid organ transplantation |
| topic | antihuman globulin complement-dependent cytotoxicity flow cytometry crossmatch human leucocyte antigen |
| url | https://journals.lww.com/10.4103/ijot.ijot_128_24 |
| work_keys_str_mv | AT shrutitapiawala unfoldingthemysteriesofcellbasedhumanleukocyteantigencrossmatchinginsolidorgantransplantation AT suchitajogale unfoldingthemysteriesofcellbasedhumanleukocyteantigencrossmatchinginsolidorgantransplantation AT dollydanielle unfoldingthemysteriesofcellbasedhumanleukocyteantigencrossmatchinginsolidorgantransplantation AT anilhandoo unfoldingthemysteriesofcellbasedhumanleukocyteantigencrossmatchinginsolidorgantransplantation |