Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs
Relevance. In the Russian Federation is noted the negative dynamics of epizootic process of brucellosis among epidemiologically important species of farm animals (cattle and small ruminants), which represents a threat to the population. Used in Russia live vaccine based on a strain of Brucella abort...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Numikom LLC
2016-08-01
|
| Series: | Эпидемиология и вакцинопрофилактика |
| Subjects: | |
| Online Access: | https://www.epidemvac.ru/jour/article/view/186 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839576416775045120 |
|---|---|
| author | L. M. Mikhailov N. L. Barannikova L. E. Tokareva S. A. Vityazeva T. P. Starovoytova V. I. Dubrovina S. V. Balakhonov |
| author_facet | L. M. Mikhailov N. L. Barannikova L. E. Tokareva S. A. Vityazeva T. P. Starovoytova V. I. Dubrovina S. V. Balakhonov |
| author_sort | L. M. Mikhailov |
| collection | DOAJ |
| description | Relevance. In the Russian Federation is noted the negative dynamics of epizootic process of brucellosis among epidemiologically important species of farm animals (cattle and small ruminants), which represents a threat to the population. Used in Russia live vaccine based on a strain of Brucella abortus 19 the BA has reduced virulence, but capable at high doses (108 -2 109 m.c.) cause a generalized infection in guinea pigs and humans, and in violation of the rules cause post-vaccination complications. Goal. Assess possibility of the thermo-extract derived from the S- and L-forms of Brucella, get an immune response in guinea pigs, and reduce the risk of infection with virulent brucella. Materials and methods. Two series of experiments were carried out on guinea pigs. Immunized guinea pigs thermo-extracts (TE) from a strain of B. abortus I-206 in the S- and L-forms, and live brucellosis vaccine (Scientific and Production Association for Immunological Preparations «Microgen», Russia). To infect guinea pigs using virulent B. abortus 544 (Reference) and B. melitensis I-203 from the museum of living cultures of the Irkutsk Scientific Research Anti-Plague Institute. Results. In the first and second experiment after immunization L TE in dose 5 mg and 10 mg after infection with B. abortus and B. melitensis 5441-203 were approximately similar results. Immunization of Brucella in TE S-form or complex of L + S TE either of two doses (5 mg or 10 mg) cultures after infection B. abortus and B. melitensis 5441-203 gave the same result as a vaccine B. abortus 19VA. Conclusions. The results indicate the prospects of further study of experimental steps for using immunizing agents TE S, L TE and TE S + L on the laboratory animals. |
| format | Article |
| id | doaj-art-0cb7a7d1e6ec4e28ab1f6d40ad420af5 |
| institution | Matheson Library |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| publishDate | 2016-08-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj-art-0cb7a7d1e6ec4e28ab1f6d40ad420af52025-08-04T13:01:59ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942016-08-01154828610.31631/2073-3046-2016-15-4-82-86185Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea PigsL. M. Mikhailov0N. L. Barannikova1L. E. Tokareva2S. A. Vityazeva3T. P. Starovoytova4V. I. Dubrovina5S. V. Balakhonov6ФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораФКУЗ «Иркутский научно-исследовательский противочумный институт» РоспотребнадзораRelevance. In the Russian Federation is noted the negative dynamics of epizootic process of brucellosis among epidemiologically important species of farm animals (cattle and small ruminants), which represents a threat to the population. Used in Russia live vaccine based on a strain of Brucella abortus 19 the BA has reduced virulence, but capable at high doses (108 -2 109 m.c.) cause a generalized infection in guinea pigs and humans, and in violation of the rules cause post-vaccination complications. Goal. Assess possibility of the thermo-extract derived from the S- and L-forms of Brucella, get an immune response in guinea pigs, and reduce the risk of infection with virulent brucella. Materials and methods. Two series of experiments were carried out on guinea pigs. Immunized guinea pigs thermo-extracts (TE) from a strain of B. abortus I-206 in the S- and L-forms, and live brucellosis vaccine (Scientific and Production Association for Immunological Preparations «Microgen», Russia). To infect guinea pigs using virulent B. abortus 544 (Reference) and B. melitensis I-203 from the museum of living cultures of the Irkutsk Scientific Research Anti-Plague Institute. Results. In the first and second experiment after immunization L TE in dose 5 mg and 10 mg after infection with B. abortus and B. melitensis 5441-203 were approximately similar results. Immunization of Brucella in TE S-form or complex of L + S TE either of two doses (5 mg or 10 mg) cultures after infection B. abortus and B. melitensis 5441-203 gave the same result as a vaccine B. abortus 19VA. Conclusions. The results indicate the prospects of further study of experimental steps for using immunizing agents TE S, L TE and TE S + L on the laboratory animals.https://www.epidemvac.ru/jour/article/view/186термоэкстрактыs- и l-формыбруцеллыиндекс инфицированностиthermo-extractss- and l-formsbrucellainfection rate |
| spellingShingle | L. M. Mikhailov N. L. Barannikova L. E. Tokareva S. A. Vityazeva T. P. Starovoytova V. I. Dubrovina S. V. Balakhonov Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs Эпидемиология и вакцинопрофилактика термоэкстракты s- и l-формы бруцеллы индекс инфицированности thermo-extracts s- and l-forms brucella infection rate |
| title | Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs |
| title_full | Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs |
| title_fullStr | Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs |
| title_full_unstemmed | Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs |
| title_short | Studying of S- and L- form Brucella’s Thermo-Extracts Immunogenic Characteristics at Cavies Guinea Pigs |
| title_sort | studying of s and l form brucella s thermo extracts immunogenic characteristics at cavies guinea pigs |
| topic | термоэкстракты s- и l-формы бруцеллы индекс инфицированности thermo-extracts s- and l-forms brucella infection rate |
| url | https://www.epidemvac.ru/jour/article/view/186 |
| work_keys_str_mv | AT lmmikhailov studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT nlbarannikova studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT letokareva studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT savityazeva studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT tpstarovoytova studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT vidubrovina studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs AT svbalakhonov studyingofsandlformbrucellasthermoextractsimmunogeniccharacteristicsatcaviesguineapigs |