Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses.
This study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading fra...
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303838&type=printable |
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| author | Diego F Quito-Avila Edison Reyes-Proaño Gerardo Armijos-Capa Ricardo I Alcalá Briseño Robert Alvarez Francisco F Flores |
| author_facet | Diego F Quito-Avila Edison Reyes-Proaño Gerardo Armijos-Capa Ricardo I Alcalá Briseño Robert Alvarez Francisco F Flores |
| author_sort | Diego F Quito-Avila |
| collection | DOAJ |
| description | This study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas. |
| format | Article |
| id | doaj-art-0c49e6e734444b718f932865943a0bae |
| institution | Matheson Library |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-0c49e6e734444b718f932865943a0bae2025-07-10T05:31:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01195e030383810.1371/journal.pone.0303838Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses.Diego F Quito-AvilaEdison Reyes-ProañoGerardo Armijos-CapaRicardo I Alcalá BriseñoRobert AlvarezFrancisco F FloresThis study presents the complete genome sequence of a novel nege-like virus identified in whiteflies (Bemisia tabaci MEAM1), provisionally designated as whitefly negevirus 1 (WfNgV1). The virus possesses a single-stranded RNA genome comprising 11,848 nucleotides, organized into four open reading frames (ORFs). These ORFs encode the putative RNA-dependent-RNA-polymerase (RdRp, ORF 1), a glycoprotein (ORF 2), a structural protein with homology to those in the SP24 family, (ORF 3), and a protein of unknown function (ORF 4). Phylogenetic analysis focusing on RdRp and SP24 amino acid sequences revealed a close relationship between WfNgV1 and Bemisia tabaci negevirus 1, a negevirus sequence recently discovered in whiteflies from Israel. Both viruses form a clade sharing a most recent common ancestor with the proposed nelorpivirus and centivirus taxa. The putative glycoprotein from ORF 2 and SP24 (ORF 3) of WfNgV1 exhibit the characteristic topologies previously reported for negevirus counterparts. This marks the first reported negevirus-like sequence from whiteflies in the Americas.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303838&type=printable |
| spellingShingle | Diego F Quito-Avila Edison Reyes-Proaño Gerardo Armijos-Capa Ricardo I Alcalá Briseño Robert Alvarez Francisco F Flores Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. PLoS ONE |
| title | Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. |
| title_full | Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. |
| title_fullStr | Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. |
| title_full_unstemmed | Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. |
| title_short | Analysis of a new negevirus-like sequence from Bemisia tabaci unveils a potential new taxon linking nelorpi- and centiviruses. |
| title_sort | analysis of a new negevirus like sequence from bemisia tabaci unveils a potential new taxon linking nelorpi and centiviruses |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0303838&type=printable |
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