Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola
Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice (Oryza sativa) production worldwide. Resistance to M. graminicola in rice could be the most effective option for its management. However, sources of germplasm with resistance to M. graminicola...
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KeAi Communications Co., Ltd.
2023-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922002817 |
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| author | Hui FENG Can-rong ZHOU Feng ZHU Xiu-hu LE De-dao JING DALY Paul Dong-mei ZHOU Li-hui WEI |
| author_facet | Hui FENG Can-rong ZHOU Feng ZHU Xiu-hu LE De-dao JING DALY Paul Dong-mei ZHOU Li-hui WEI |
| author_sort | Hui FENG |
| collection | DOAJ |
| description | Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice (Oryza sativa) production worldwide. Resistance to M. graminicola in rice could be the most effective option for its management. However, sources of germplasm with resistance to M. graminicola in rice remain limited. Here, we describe the root attraction, gall formation and genetic analysis of the resistance to M. graminicola in the rice variety Huidao 5. A nematode attraction assay showed that second-stage juveniles (J2s) of M. graminicola were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing 9108. Microscopic observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation, but their subsequent movement to the root tip was hindered in Huaidao 5, resulting in decreased nematode number compared to Nanjing 9108. Additionally, we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively, and found that gall number was significantly reduced, and nematode development was clearly suppressed in Huaidao 5. To investigate the genetic basis of this resistance, cross breeding was performed between the Huaidao 5 and Nanjing 9108 varieties. There was no reduction in the resistance of the F1 offspring to M. graminicola in the greenhouse or field trials, suggesting that a dominant gene could control resistance in Huaidao 5. In summary, this study provides a detailed characterization of a novel source of resistance to M. graminicola in rice, which is of great potential for use in crop breeding. |
| format | Article |
| id | doaj-art-08f26d077a3b49d284c037f12a9be3f9 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-08f26d077a3b49d284c037f12a9be3f92025-07-02T01:07:35ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-10-01221030813089Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicolaHui FENG0Can-rong ZHOU1Feng ZHU2Xiu-hu LE3De-dao JING4DALY Paul5Dong-mei ZHOU6Li-hui WEI7Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.ChinaPlant Protection and Quarantine Station of Jiangsu Province, Nanjing 210009, P.R.ChinaSchool of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, P.R.ChinaZhenjiang Institute of Agricultural Science in Hilly Area of Jiangsu Province, Jurong 212400, P.R.ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.ChinaInstitute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.China; Correspondence WEI Li-hui, Tel: +86-25-84390783Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice (Oryza sativa) production worldwide. Resistance to M. graminicola in rice could be the most effective option for its management. However, sources of germplasm with resistance to M. graminicola in rice remain limited. Here, we describe the root attraction, gall formation and genetic analysis of the resistance to M. graminicola in the rice variety Huidao 5. A nematode attraction assay showed that second-stage juveniles (J2s) of M. graminicola were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing 9108. Microscopic observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation, but their subsequent movement to the root tip was hindered in Huaidao 5, resulting in decreased nematode number compared to Nanjing 9108. Additionally, we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively, and found that gall number was significantly reduced, and nematode development was clearly suppressed in Huaidao 5. To investigate the genetic basis of this resistance, cross breeding was performed between the Huaidao 5 and Nanjing 9108 varieties. There was no reduction in the resistance of the F1 offspring to M. graminicola in the greenhouse or field trials, suggesting that a dominant gene could control resistance in Huaidao 5. In summary, this study provides a detailed characterization of a novel source of resistance to M. graminicola in rice, which is of great potential for use in crop breeding.http://www.sciencedirect.com/science/article/pii/S2095311922002817root-knot nematodeMeloidogyne graminicolariceresistance |
| spellingShingle | Hui FENG Can-rong ZHOU Feng ZHU Xiu-hu LE De-dao JING DALY Paul Dong-mei ZHOU Li-hui WEI Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola Journal of Integrative Agriculture root-knot nematode Meloidogyne graminicola rice resistance |
| title | Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola |
| title_full | Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola |
| title_fullStr | Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola |
| title_full_unstemmed | Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola |
| title_short | Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola |
| title_sort | resistance analysis of the rice variety huaidao 5 against root knot nematode meloidogyne graminicola |
| topic | root-knot nematode Meloidogyne graminicola rice resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922002817 |
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