Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis
Introduction and objective: Assessment of α-synuclein (αSyn) seed amplification assays (αSyn-SAA) accuracy in distinguishing Parkinson’s disease (PD) from controls using cerebrospinal fluid (CSF), blood, skin, extracellular vesicles (ECV), saliva, olfactory mucosa (OM), gastrointestinal tract (GIT),...
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MDPI AG
2025-06-01
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| Series: | Clinics and Practice |
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| Online Access: | https://www.mdpi.com/2039-7283/15/6/107 |
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| author | Jamir Pitton Rissardo Ana Leticia Fornari Caprara |
| author_facet | Jamir Pitton Rissardo Ana Leticia Fornari Caprara |
| author_sort | Jamir Pitton Rissardo |
| collection | DOAJ |
| description | Introduction and objective: Assessment of α-synuclein (αSyn) seed amplification assays (αSyn-SAA) accuracy in distinguishing Parkinson’s disease (PD) from controls using cerebrospinal fluid (CSF), blood, skin, extracellular vesicles (ECV), saliva, olfactory mucosa (OM), gastrointestinal tract (GIT), and submandibular gland (SMG). Methodology: PubMed was searched for articles from 2010 to January 2025. The quality assessment used robvis. Diagnostic values with a 95% confidence interval (CI) were obtained. Z-test, Wald CI, and ANOVA were performed. Diagnostic odds ratio (DOR) was used. Results: αSyn-SAAs showed strong diagnostic performance in distinguishing PD from controls across various tissue and fluid types. Overall, αSyn-SAAs demonstrated high sensitivity (86%) and specificity (92%). Among all biomatrices, CSF, skin, blood, and ECV yielded the highest diagnostic accuracy, with sensitivity and specificity approaching or exceeding 90%. In contrast, saliva, oral mucosa, and gastrointestinal tract samples showed more modest sensitivity, though specificity remained relatively high. ECV, CSF, skin, and blood matrices also demonstrated the highest DOR, supporting their potential clinical utility. Conclusions: ECV and blood warrant priority in αSyn-SAA for high accuracy and minimal invasiveness, while GIT, OM, and oral samples show limited utility; saliva and SMG need refinement. |
| format | Article |
| id | doaj-art-08e611e6e16147e590c20bfd1c1c17d7 |
| institution | Matheson Library |
| issn | 2039-7283 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Clinics and Practice |
| spelling | doaj-art-08e611e6e16147e590c20bfd1c1c17d72025-06-25T13:39:09ZengMDPI AGClinics and Practice2039-72832025-06-0115610710.3390/clinpract15060107Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-AnalysisJamir Pitton Rissardo0Ana Leticia Fornari Caprara1Neurology Department, Cooper University Hospital, Camden, NJ 08103, USANeurology Department, Cooper University Hospital, Camden, NJ 08103, USAIntroduction and objective: Assessment of α-synuclein (αSyn) seed amplification assays (αSyn-SAA) accuracy in distinguishing Parkinson’s disease (PD) from controls using cerebrospinal fluid (CSF), blood, skin, extracellular vesicles (ECV), saliva, olfactory mucosa (OM), gastrointestinal tract (GIT), and submandibular gland (SMG). Methodology: PubMed was searched for articles from 2010 to January 2025. The quality assessment used robvis. Diagnostic values with a 95% confidence interval (CI) were obtained. Z-test, Wald CI, and ANOVA were performed. Diagnostic odds ratio (DOR) was used. Results: αSyn-SAAs showed strong diagnostic performance in distinguishing PD from controls across various tissue and fluid types. Overall, αSyn-SAAs demonstrated high sensitivity (86%) and specificity (92%). Among all biomatrices, CSF, skin, blood, and ECV yielded the highest diagnostic accuracy, with sensitivity and specificity approaching or exceeding 90%. In contrast, saliva, oral mucosa, and gastrointestinal tract samples showed more modest sensitivity, though specificity remained relatively high. ECV, CSF, skin, and blood matrices also demonstrated the highest DOR, supporting their potential clinical utility. Conclusions: ECV and blood warrant priority in αSyn-SAA for high accuracy and minimal invasiveness, while GIT, OM, and oral samples show limited utility; saliva and SMG need refinement.https://www.mdpi.com/2039-7283/15/6/107alpha-synucleinsynucleinopathybiomarkerbiofluidprotein misfolding cyclic amplification (PMCA)real-time quaking-induced conversion (RT-QuIC) |
| spellingShingle | Jamir Pitton Rissardo Ana Leticia Fornari Caprara Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis Clinics and Practice alpha-synuclein synucleinopathy biomarker biofluid protein misfolding cyclic amplification (PMCA) real-time quaking-induced conversion (RT-QuIC) |
| title | Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis |
| title_full | Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis |
| title_fullStr | Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis |
| title_full_unstemmed | Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis |
| title_short | Alpha-Synuclein Seed Amplification Assays in Parkinson’s Disease: A Systematic Review and Network Meta-Analysis |
| title_sort | alpha synuclein seed amplification assays in parkinson s disease a systematic review and network meta analysis |
| topic | alpha-synuclein synucleinopathy biomarker biofluid protein misfolding cyclic amplification (PMCA) real-time quaking-induced conversion (RT-QuIC) |
| url | https://www.mdpi.com/2039-7283/15/6/107 |
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