<i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers

<i>Lactobacillus helveticus R0052</i> and <i>Bifidobacterium longum R0175</i> Supplementation: An Exploratory, Randomized, Placebo-Controlled Trial of Endocannabinoid and Inflammatory Responses in Female Dancers

The anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with <i>Lactobacillus helveti...

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Main Authors: Jakub Wiącek, Karolina Skonieczna-Żydecka, Igor Łoniewski, Chariklia K. Deli, Ioannis G. Fatouros, Athanasios Z. Jamurtas, Dominika Moszczyńska, Joanna Karolkiewicz
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Microorganisms
Subjects:
endocannabinoid system
gut microbiome
anandamide
lipopolysaccharide
probiotics
dancers
Online Access:https://www.mdpi.com/2076-2607/13/6/1284
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Summary:The anandamide (AEA) and lipopolysaccharide (LPS) interaction is gaining attention, but evidence on the influence of probiotics on endocannabinoid system (ECS) biomarkers remains limited. This study (NCT05567653) investigated the effects of 12-week supplementation with <i>Lactobacillus helveticus</i> R0052 and <i>Bifidobacterium longum</i> R0175 on AEA (main outcome) and inflammatory biomarkers in female dancers. Fifteen participants (5 probiotic, 10 placebo) were included in the final analysis. Serum levels of AEA, LPS, and cytokines (tumor necrosis factor-alpha—TNF-α, interleukin-1 beta—IL-1β, and interleukin-10—IL-10) were measured using an ELISA (enzyme-linked immunosorbent assay), and the psychological stress responses were evaluated using the Mini-COPE questionnaire. At the baseline, a correlation between AEA and LPS was observed (Spearman’s r = 0.9677, <i>p</i> < 0.05). After 12 weeks, no statistically significant differences in the AEA, LPS, cytokine levels, or stress-coping strategies were observed between the probiotic and placebo groups (LPS–probiotic: +3.48 EU/L, <i>p</i> = 0.9361; placebo: +56.98 EU/L, <i>p</i> = 0.0694; AEA–probiotic: −1.11 ng/mL, <i>p</i> = 0.9538; placebo: +14.08 ng/mL, <i>p</i> = 0.4749). The direction of change may indicate a trend toward increased inflammation in the absence of probiotics, consistent with patterns described in previous literature. However, these results should be viewed as hypothesis generating and warrant confirmation in larger trials.
ISSN:2076-2607

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