In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption
In vitro fertilization is used to produce embryos from high-genetic-merit cattle. However, these embryos often exhibit inferior quality than those derived in vivo, possibly due to increased oxidative stress. This study investigates whether adding antioxidant polyphenols (resveratrol (RV), chlorogeni...
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MDPI AG
2025-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/6/621 |
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| author | Katrin Giller Dominique Schmid Idil Serbetci Manuel Meleán Sarah Greve Ferdinand von Meyenn Heinrich Bollwein Carolina Herrera |
| author_facet | Katrin Giller Dominique Schmid Idil Serbetci Manuel Meleán Sarah Greve Ferdinand von Meyenn Heinrich Bollwein Carolina Herrera |
| author_sort | Katrin Giller |
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| description | In vitro fertilization is used to produce embryos from high-genetic-merit cattle. However, these embryos often exhibit inferior quality than those derived in vivo, possibly due to increased oxidative stress. This study investigates whether adding antioxidant polyphenols (resveratrol (RV), chlorogenic acid (CA), ellagic acid (EA)) to the in vitro maturation (IVM) medium at 0.25, 0.5, and 1 μM could improve embryo development. Oxygen consumption and gene expression were evaluated at the blastocyst stage following treatment with 1 μM of each polyphenol. Embryo development (cleavage, blastocyst, and hatched blastocyst rates) and oxygen consumption were not significantly affected by polyphenols. However, RV significantly upregulated the mRNA expression of the antioxidant enzyme glutathione peroxidase 4 (GPX4), while GPX4 expression was significantly downregulated by EA. Expression of other gene markers related to antioxidant defense, apoptosis, development, and metabolism was not significantly affected. The results indicate that applying RV, CA, and EA during bovine oocyte IVM does not enhance in vitro embryo development at the tested concentrations. Given the opposing effects of RV and EA on the expression of GPX4, the effects of those polyphenols regarding the protection of embryos from oxidative stress and potential long-term effects on the offspring remain to be elucidated. |
| format | Article |
| id | doaj-art-06e36701cde4417d85ede410aa015be0 |
| institution | Matheson Library |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-06e36701cde4417d85ede410aa015be02025-06-25T13:23:04ZengMDPI AGAntioxidants2076-39212025-05-0114662110.3390/antiox14060621In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen ConsumptionKatrin Giller0Dominique Schmid1Idil Serbetci2Manuel Meleán3Sarah Greve4Ferdinand von Meyenn5Heinrich Bollwein6Carolina Herrera7Department of Molecular Nutritional Science, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, GermanyAnimal Nutrition, ETH Zurich, Universitaetstrasse 2, 8092 Zurich, SwitzerlandAgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, SwitzerlandAgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, SwitzerlandDepartment of Molecular Nutritional Science, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, GermanyLaboratory of Nutrition and Metabolic Epigenetics, ETH Zurich, Schorenstrasse 16, 8603 Schwerzenbach, SwitzerlandAgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, SwitzerlandAgroVet-Strickhof, University of Zurich, Eschikon 27, 8315 Lindau, SwitzerlandIn vitro fertilization is used to produce embryos from high-genetic-merit cattle. However, these embryos often exhibit inferior quality than those derived in vivo, possibly due to increased oxidative stress. This study investigates whether adding antioxidant polyphenols (resveratrol (RV), chlorogenic acid (CA), ellagic acid (EA)) to the in vitro maturation (IVM) medium at 0.25, 0.5, and 1 μM could improve embryo development. Oxygen consumption and gene expression were evaluated at the blastocyst stage following treatment with 1 μM of each polyphenol. Embryo development (cleavage, blastocyst, and hatched blastocyst rates) and oxygen consumption were not significantly affected by polyphenols. However, RV significantly upregulated the mRNA expression of the antioxidant enzyme glutathione peroxidase 4 (GPX4), while GPX4 expression was significantly downregulated by EA. Expression of other gene markers related to antioxidant defense, apoptosis, development, and metabolism was not significantly affected. The results indicate that applying RV, CA, and EA during bovine oocyte IVM does not enhance in vitro embryo development at the tested concentrations. Given the opposing effects of RV and EA on the expression of GPX4, the effects of those polyphenols regarding the protection of embryos from oxidative stress and potential long-term effects on the offspring remain to be elucidated.https://www.mdpi.com/2076-3921/14/6/621polyphenolantioxidant enzymesblastocyst ratehatching ratedevelopmental competencedairy cow |
| spellingShingle | Katrin Giller Dominique Schmid Idil Serbetci Manuel Meleán Sarah Greve Ferdinand von Meyenn Heinrich Bollwein Carolina Herrera In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption Antioxidants polyphenol antioxidant enzymes blastocyst rate hatching rate developmental competence dairy cow |
| title | In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption |
| title_full | In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption |
| title_fullStr | In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption |
| title_full_unstemmed | In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption |
| title_short | In Vitro Maturation of Bovine Oocytes in the Presence of Resveratrol and Ellagic Acid but Not Chlorogenic Acid Modulates Blastocyst Antioxidant Gene Expression Without Impacting Embryo Development and Oxygen Consumption |
| title_sort | in vitro maturation of bovine oocytes in the presence of resveratrol and ellagic acid but not chlorogenic acid modulates blastocyst antioxidant gene expression without impacting embryo development and oxygen consumption |
| topic | polyphenol antioxidant enzymes blastocyst rate hatching rate developmental competence dairy cow |
| url | https://www.mdpi.com/2076-3921/14/6/621 |
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