Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats
Dillenia ochreata is a traditional medicine used to treat wounds and scabies. In wound healing, one of the treatments is to reduce pain (analgesic). Some compounds of triterpenoid groups have been known to be active as analgesic compounds. The leaf of D. ochrea was reported to contain secondary met...
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Universitas Jambi
2025-06-01
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| Series: | Chempublish Journal |
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| Online Access: | https://online-journal.unja.ac.id/chp/article/view/43080 |
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| author | Heni Yohandini Fahdelaa Ghaniya Muharni Muharni Fitrya Fitrya |
| author_facet | Heni Yohandini Fahdelaa Ghaniya Muharni Muharni Fitrya Fitrya |
| author_sort | Heni Yohandini |
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Dillenia ochreata is a traditional medicine used to treat wounds and scabies. In wound healing, one of the treatments is to reduce pain (analgesic). Some compounds of triterpenoid groups have been known to be active as analgesic compounds. The leaf of D. ochrea was reported to contain secondary metabolites triterpenoid centulic acid and 3β-glucopyranosyl-lup-20(29)-en-28 oat. This study aimed to evaluate the analgesic activity and standardization of the ethanol extract of D. ochreata leaf and determine the mechanism of action. Analgesic activity was determined by a hot plate method and formalin test, and the mechanism of action was through muscarinic, dopamine, and opiate receptors, standardization of extract using the method issued by the Indonesian Ministry of Health. The ethanol extract of D. ochreata leaf at a 400 mg/kg bw dose has higher analgesic activity (24.85%) than the positive control (18.32%). Statistical analysis showed a significant difference in analgesic activity percentage between the positive group and those with a 400 mg/kg bw group dose. The 400 mg/kg bw dose also showed a significant difference (p<0.05) between neurogenic pain (46.92%) and inflammation (61.19%) in the formalin test caused by opioid receptors. The evaluation of the analgesic mechanism showed the ethanol extract of D. ochreata leaf works through opioid receptors. The extract meets the requirements of the standard parameters. The leaf extract of D. ochreata can be developed as an anti-analgesic from natural medicine.
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| format | Article |
| id | doaj-art-0692c842cfd94038bd73b64c7a2bbf2c |
| institution | Matheson Library |
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| language | Indonesian |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-0692c842cfd94038bd73b64c7a2bbf2c2025-07-09T14:15:19ZindUniversitas JambiChempublish Journal2503-45882025-06-019110.22437/chp.v9i1.43080Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar RatsHeni Yohandini0Fahdelaa Ghaniya1Muharni Muharni2Fitrya Fitrya3Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatera, 30662 IndonesiaDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatera, 30662 IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatera, 30662 IndonesiaDepartment of Pharmacy, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, Ogan Ilir, South Sumatera, 30662 Indonesia Dillenia ochreata is a traditional medicine used to treat wounds and scabies. In wound healing, one of the treatments is to reduce pain (analgesic). Some compounds of triterpenoid groups have been known to be active as analgesic compounds. The leaf of D. ochrea was reported to contain secondary metabolites triterpenoid centulic acid and 3β-glucopyranosyl-lup-20(29)-en-28 oat. This study aimed to evaluate the analgesic activity and standardization of the ethanol extract of D. ochreata leaf and determine the mechanism of action. Analgesic activity was determined by a hot plate method and formalin test, and the mechanism of action was through muscarinic, dopamine, and opiate receptors, standardization of extract using the method issued by the Indonesian Ministry of Health. The ethanol extract of D. ochreata leaf at a 400 mg/kg bw dose has higher analgesic activity (24.85%) than the positive control (18.32%). Statistical analysis showed a significant difference in analgesic activity percentage between the positive group and those with a 400 mg/kg bw group dose. The 400 mg/kg bw dose also showed a significant difference (p<0.05) between neurogenic pain (46.92%) and inflammation (61.19%) in the formalin test caused by opioid receptors. The evaluation of the analgesic mechanism showed the ethanol extract of D. ochreata leaf works through opioid receptors. The extract meets the requirements of the standard parameters. The leaf extract of D. ochreata can be developed as an anti-analgesic from natural medicine. https://online-journal.unja.ac.id/chp/article/view/43080Analgesic Dillenia ochreataformalin testhot plate testreceptors |
| spellingShingle | Heni Yohandini Fahdelaa Ghaniya Muharni Muharni Fitrya Fitrya Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats Chempublish Journal Analgesic Dillenia ochreata formalin test hot plate test receptors |
| title | Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats |
| title_full | Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats |
| title_fullStr | Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats |
| title_full_unstemmed | Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats |
| title_short | Analgesic Effect of Ethanol Extract of Dillenia ochreata (Miq.) Teijsm. & Binn. Ex Martelli in Wistar Rats |
| title_sort | analgesic effect of ethanol extract of dillenia ochreata miq teijsm binn ex martelli in wistar rats |
| topic | Analgesic Dillenia ochreata formalin test hot plate test receptors |
| url | https://online-journal.unja.ac.id/chp/article/view/43080 |
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