Sequence and phylogenetic analysis of chicken reoviruses in China
Avian reovirus (ARV) has been responsible for many cases of chicken tenosynovitis in China in recent years, causing high morbidity among layer and broiler chickens. To study the degree of genetic divergence and evolution among ARVs, the full-length nucleotide sequences of the σC-encoding gene of eig...
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KeAi Communications Co., Ltd.
2016-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191561200X |
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| author | Chu WEN Qi ZHONG Jia-dong ZHANG Jian-shan LU Li-xin ZHANG Xi-min YUAN Meng-hou GAN Xue-peng CAI Guo-zhong ZHANG |
| author_facet | Chu WEN Qi ZHONG Jia-dong ZHANG Jian-shan LU Li-xin ZHANG Xi-min YUAN Meng-hou GAN Xue-peng CAI Guo-zhong ZHANG |
| author_sort | Chu WEN |
| collection | DOAJ |
| description | Avian reovirus (ARV) has been responsible for many cases of chicken tenosynovitis in China in recent years, causing high morbidity among layer and broiler chickens. To study the degree of genetic divergence and evolution among ARVs, the full-length nucleotide sequences of the σC-encoding gene of eight ARV field isolates and the entire coding-region sequences of four isolates were determined and analyzed. The sequence analysis revealed that the eight σC-encoding genes shared 99.0–99.9% nucleotide sequence identity with each other and over 99% with the chicken reovirus reference strain S1133. However, the nucleotide sequences of the eight σC-encoding genes varied extensively from that of isolate AVS-B (GenBank accession no. FR694197), with only 55.5% identity. A sequence analysis of the whole ARV-coding region showed some nucleotide substitutions in the open reading frames encoding λA, λB, λC, μA, μB, μNS, σC, σA, σB, and σNS in the field strains. A phylogenetic analysis showed that all eight isolates clustered in group I with S1133, but that four field isolates shared less homology with strain S1133 than the others, indicating that they had been evolved in the field. We also studied the pathogenicity of two strains. No characteristic lesions were observed in vaccinated chickens, and no virus was detected in sampled tissues. However, an enzyme-linked immunosorbent assay revealed significant differences between the antibody responses of the inoculated groups and the negative controls. These results revealed that Chinese isolates shared the highest sequence homologies with S1133, grouped together in one cluster. Although the vaccination against ARV is used in farms, the pathogens still persist in Chinese poultry flocks. |
| format | Article |
| id | doaj-art-067f19a3e69c46d7bd8f7a58c478c7e9 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-067f19a3e69c46d7bd8f7a58c478c7e92025-07-02T00:48:42ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-08-0115818461855Sequence and phylogenetic analysis of chicken reoviruses in ChinaChu WEN0Qi ZHONG1Jia-dong ZHANG2Jian-shan LU3Li-xin ZHANG4Xi-min YUAN5Meng-hou GAN6Xue-peng CAI7Guo-zhong ZHANG8Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China; WEN ChuKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. ChinaBeijing Kang Mu Animal Health Products and Veterinary Apparatus Supply Center, Beijing 100085, P.R. ChinaBeijing Kang Mu Animal Health Products and Veterinary Apparatus Supply Center, Beijing 100085, P.R. ChinaBeijing Kang Mu Animal Health Products and Veterinary Apparatus Supply Center, Beijing 100085, P.R. ChinaBeijing Kang Mu Animal Health Products and Veterinary Apparatus Supply Center, Beijing 100085, P.R. ChinaKey Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. ChinaChina Institute of Veterinary Drug Control, Beijing 100081, P.R. China; Correspondence CAI Xue-peng, Tel: +86-10-62158844, Fax: +86-10-62103602Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China; ZHANG Guo-zhong, Tel: +86-10-62733660, Fax: +86-10-62732984Avian reovirus (ARV) has been responsible for many cases of chicken tenosynovitis in China in recent years, causing high morbidity among layer and broiler chickens. To study the degree of genetic divergence and evolution among ARVs, the full-length nucleotide sequences of the σC-encoding gene of eight ARV field isolates and the entire coding-region sequences of four isolates were determined and analyzed. The sequence analysis revealed that the eight σC-encoding genes shared 99.0–99.9% nucleotide sequence identity with each other and over 99% with the chicken reovirus reference strain S1133. However, the nucleotide sequences of the eight σC-encoding genes varied extensively from that of isolate AVS-B (GenBank accession no. FR694197), with only 55.5% identity. A sequence analysis of the whole ARV-coding region showed some nucleotide substitutions in the open reading frames encoding λA, λB, λC, μA, μB, μNS, σC, σA, σB, and σNS in the field strains. A phylogenetic analysis showed that all eight isolates clustered in group I with S1133, but that four field isolates shared less homology with strain S1133 than the others, indicating that they had been evolved in the field. We also studied the pathogenicity of two strains. No characteristic lesions were observed in vaccinated chickens, and no virus was detected in sampled tissues. However, an enzyme-linked immunosorbent assay revealed significant differences between the antibody responses of the inoculated groups and the negative controls. These results revealed that Chinese isolates shared the highest sequence homologies with S1133, grouped together in one cluster. Although the vaccination against ARV is used in farms, the pathogens still persist in Chinese poultry flocks.http://www.sciencedirect.com/science/article/pii/S209531191561200Xavian reoviruschickengenomeS1homologyphylogenetic analysis |
| spellingShingle | Chu WEN Qi ZHONG Jia-dong ZHANG Jian-shan LU Li-xin ZHANG Xi-min YUAN Meng-hou GAN Xue-peng CAI Guo-zhong ZHANG Sequence and phylogenetic analysis of chicken reoviruses in China Journal of Integrative Agriculture avian reovirus chicken genome S1 homology phylogenetic analysis |
| title | Sequence and phylogenetic analysis of chicken reoviruses in China |
| title_full | Sequence and phylogenetic analysis of chicken reoviruses in China |
| title_fullStr | Sequence and phylogenetic analysis of chicken reoviruses in China |
| title_full_unstemmed | Sequence and phylogenetic analysis of chicken reoviruses in China |
| title_short | Sequence and phylogenetic analysis of chicken reoviruses in China |
| title_sort | sequence and phylogenetic analysis of chicken reoviruses in china |
| topic | avian reovirus chicken genome S1 homology phylogenetic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S209531191561200X |
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