Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection

Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection

The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects. In the construction of CRISPR/Cas9-mediated dsLmRNase2–/– mutant locusts, we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibi...

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Bibliographic Details
Main Authors: Ting-ting ZHANG, Ting-mei WEN, Yang YUE, Qiang YAN, Er-xia DU, San-hong FAN, Siegfried ROTH, Sheng LI, Jian-zhen ZHANG, Xue-yao ZHANG, Min ZHANG
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2021-10-01
Series:Journal of Integrative Agriculture
Subjects:
brown tanned eggs
CRISPR/Cas9
mutant locusts
microinjection
defense ability
Online Access:http://www.sciencedirect.com/science/article/pii/S209531192163736X
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