Triple-mode point-of-care diagnostics for high-risk human papillomavirus in urine
Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal am...
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| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Artikel |
| Språk: | engelska |
| Publicerad: |
Elsevier
2025-10-01
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| Serie: | Biosensors and Bioelectronics: X |
| Ämnen: | |
| Länkar: | http://www.sciencedirect.com/science/article/pii/S2590137025000858 |
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| Sammanfattning: | Human papillomavirus (HPV) screening is crucial for early diagnosis and prevention of cervical cancer, yet fast and convenient HPV detection remains challenging, especially in resource-limited areas. Herein, we developed a nucleic acid test named SSMG-LAMP, which combined loop-mediated isothermal amplification (LAMP) with an engineered DNA indicator (SYBR Safe – Malachite Green) for the point-of-care diagnosis of high-risk HPV strains (HPV 16 and 18) in urine. The assay can be completed within 45 min, including DNA extraction, SSMG-LAMP reaction, and signal readout using a simple, portable system. This system enabled the triple-mode detection of DNA targets using colorimetry, fluorometry, and electrochemistry, and can detect as low as 10 copies μL−1 HPV DNA. As a preliminary validation, we used SSMG-LAMP for a blind test of 16 clinical urine samples to detect HPV 16 and 18, and results showed a sensitivity of >80 % and specificity of up to 96.2 %, with a 95 % confidence interval. This triple-mode HPV detection strategy holds potential for point-of-care cervical cancer screening in low-resource settings. |
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| ISSN: | 2590-1370 |