Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival
Macrophages are the primary immune cells in the cochlea, essential for maintaining cochlear homeostasis and orchestrating inflammatory responses to pathological events. Although these cells have been found in various parts of the cochlea, their presence in the organ of Corti, a critical structure fo...
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Frontiers Media S.A.
2025-06-01
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| 叢編: | Frontiers in Immunology |
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| 在線閱讀: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1617146/full |
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| author | Mengxiao Ye Celia Zhang Dalian Ding Guang-Di Chen Henry J. Adler Rania Sharaf Bo Hua Hu |
| author_facet | Mengxiao Ye Celia Zhang Dalian Ding Guang-Di Chen Henry J. Adler Rania Sharaf Bo Hua Hu |
| author_sort | Mengxiao Ye |
| collection | DOAJ |
| description | Macrophages are the primary immune cells in the cochlea, essential for maintaining cochlear homeostasis and orchestrating inflammatory responses to pathological events. Although these cells have been found in various parts of the cochlea, their presence in the organ of Corti, a critical structure for acoustic sensing, remains poorly understood. The present study was designed to examine macrophage responses to ototoxic drug-induced cochlear damage and age-related cochlear degeneration, with a particular focus on the pathological conditions that trigger macrophage recruitment into the organ of Corti. We used a model of ototoxicity induced by cyclodextrin, a cyclic oligosaccharide known for its ability to induce rapid sensory cell damage at high doses. Cochlear tissues were collected for macrophage assessment. Multiple protein markers, including CD45, Iba1, galectin-3, CD68, myosin 7α, and IFIT3, were used to label sensory cells, macrophages, and supporting cells in cochlear sensory epithelia. Consistent with previous reports, our study confirms that macrophages are absent from the organ of Corti in mouse cochleae under normal conditions and during the acute phase of cochlear damage. However, macrophages enter the organ of Corti during the chronic phase of cochlear pathogenesis. These macrophages exhibit an activated state and display a distinct functional profile compared to macrophages outside the organ of Corti. Importantly, we demonstrate that the organ of Corti macrophage activity is not directly related to the process of sensory cell degradation. Instead, their activity is associated with supporting cell pathogenesis. Moreover, our study shows that the organ of Corti macrophages are also present in aging mouse cochleae. Collectively, our findings reveal the conditions that lead to macrophage recruitment into the organ of Corti and their involvement in supporting cell survival and degeneration. These findings provide valuable insights for future strategies focused on modulating macrophage activity to reduce tissue damage and promote repair in the cochlea following injury. |
| format | Article |
| id | doaj-art-036bcedade7440098fbbdc57ecab17a6 |
| institution | Matheson Library |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-036bcedade7440098fbbdc57ecab17a62025-06-25T04:10:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.16171461617146Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survivalMengxiao Ye0Celia Zhang1Dalian Ding2Guang-Di Chen3Henry J. Adler4Rania Sharaf5Bo Hua Hu6Department of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY, United StatesDepartment of Audiology, School of Health Sciences, University of the Pacific, San Francisco, CA, United StatesDepartment of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY, United StatesDepartment of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY, United StatesDepartment of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY, United StatesDepartment of Communicative Disorders and Sciences, University at Buffalo, Buffalo, NY, United StatesDepartment of Speech, Language and Hearing, University of Texas at Dallas, Dallas, TX, United StatesMacrophages are the primary immune cells in the cochlea, essential for maintaining cochlear homeostasis and orchestrating inflammatory responses to pathological events. Although these cells have been found in various parts of the cochlea, their presence in the organ of Corti, a critical structure for acoustic sensing, remains poorly understood. The present study was designed to examine macrophage responses to ototoxic drug-induced cochlear damage and age-related cochlear degeneration, with a particular focus on the pathological conditions that trigger macrophage recruitment into the organ of Corti. We used a model of ototoxicity induced by cyclodextrin, a cyclic oligosaccharide known for its ability to induce rapid sensory cell damage at high doses. Cochlear tissues were collected for macrophage assessment. Multiple protein markers, including CD45, Iba1, galectin-3, CD68, myosin 7α, and IFIT3, were used to label sensory cells, macrophages, and supporting cells in cochlear sensory epithelia. Consistent with previous reports, our study confirms that macrophages are absent from the organ of Corti in mouse cochleae under normal conditions and during the acute phase of cochlear damage. However, macrophages enter the organ of Corti during the chronic phase of cochlear pathogenesis. These macrophages exhibit an activated state and display a distinct functional profile compared to macrophages outside the organ of Corti. Importantly, we demonstrate that the organ of Corti macrophage activity is not directly related to the process of sensory cell degradation. Instead, their activity is associated with supporting cell pathogenesis. Moreover, our study shows that the organ of Corti macrophages are also present in aging mouse cochleae. Collectively, our findings reveal the conditions that lead to macrophage recruitment into the organ of Corti and their involvement in supporting cell survival and degeneration. These findings provide valuable insights for future strategies focused on modulating macrophage activity to reduce tissue damage and promote repair in the cochlea following injury.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1617146/fullmacrophagecochleaorgan of Cortisupporting cellsinflammationgalectin-3 |
| spellingShingle | Mengxiao Ye Celia Zhang Dalian Ding Guang-Di Chen Henry J. Adler Rania Sharaf Bo Hua Hu Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival Frontiers in Immunology macrophage cochlea organ of Corti supporting cells inflammation galectin-3 |
| title | Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| title_full | Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| title_fullStr | Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| title_full_unstemmed | Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| title_short | Organ of Corti macrophages: a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| title_sort | organ of corti macrophages a distinct group of cochlear macrophages with potential roles in supporting cell degeneration and survival |
| topic | macrophage cochlea organ of Corti supporting cells inflammation galectin-3 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1617146/full |
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